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:: Volume 23, Issue 2 (Bimonthly 2019) ::
Feyz 2019, 23(2): 135-142 Back to browse issues page
Evaluation of Beclin-1 and Atg5 genes expression levels in peripheral blood cells of patients with rheumatoid arthritis
Bahareh Kardideh , Mona Sadeghalvad , Zahra Samimi , Hamid Reza Mohammadi Motlagh , Mahdi Taghadosi *
Department of Immunology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, I. R. Iran. , mtaghad@gmail.com
Abstract:   (2031 Views)
Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease, which primarily affects the joints. During RA development, T cells and other immune cells are recruited to the synovial tissue and promote RA. Autophagy is a process in which intracellular organelles and compounds are degraded. Autophagy as a regulator of cell homeostasis can affect immune cells activation and contribute in RA pathogenesis. The aim of this study was to evaluate the autophagy-related genes (Atgs) expression in two groups of RA patients and healthy subjects.
Materials and Methods: Peripheral blood was obtained from three groups of donors including 20 patients with early RA, 20 undertreatment RA patients (with methotrexate, hydroxychloroquine and prednisolone therapy) and 20 age- and sex-matched healthy subjects. The expression of two autophagy-related genes was investigated by the real-time PCR technique.
Results: The Beclin-1 expression showed a 3.41-fold increase in the patients with early RA compared to healthy subjects but in the under treatment patients it was 1.5 times higher than healthy persons (P<0.05). The Atg5 gene expression in the early RA patients increased by 2.4 times more than healthy subjects (P<0.05).
Conclusion: The findings of this study show that in early RA patients, the increased expression of Atgs can promote RA pathogenesis. Also, findings suggest that the decreased autophagy can reduce RA severity.
Keywords: Autophagy, Autophagy related genes, Rheumatoid arthritis, Atg5, Beclin-1
Full-Text [PDF 327 kb]   (638 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2018/10/24 | Accepted: 2019/02/26 | Published: 2019/06/10
1. Mcinnes IB, Schett G. The Pathogenesis of Rheumatoid Arthritis. N Engl J Med 2011; 365(23): 2205-19.
2. Zafari P, Sh M, Iranshahi N, Jalili C, Taghadosi M. Relationship between vitamin D plasma level and Foxp3 gene expression among rheumatoid arthritis patients Foxp3. Sci J Kurdistan Univ Med Sci 2018; (92): 40-8. [in Persian]
3. Mcinnes IB, Schett G. Pathogenetic insights from the treatment of rheumatoid arthritis. Lancet 2017; 389(10086): 2328–37.
4. Smolen JS, Aletaha D, Mcinnes IB. Rheumatoid arthritis. Lancet 2016; 388(10055): 2023–38.
5. Cope AP. Review T cells in rheumatoid arthritis. Arthritis Res Ther 2008; 10 Suppl 1: S1.
6. Ravikumar B, Sarkar S, Davies JE, Futter M, Garcia-arencibia M, Green-thompson ZW, et al. Regulation of Mammalian Autophagy in Physiology and Pathophysiology. Physiol Rev 2010; 90(40): 1383–435.
7. Naito T, Tanaka H, Naoe Y, Taniuchi I. Transcriptional control of T-cell development. Int Immunol 2018; 23(11): 661–8.
8. Vomero M, Barbati C, Colasanti T, Perricone C, Novelli L, Ceccarelli F, et al. Autophagy and Rheumatoid Arthritis: Current Knowledges and Future Perspectives. Front Immunol 2018; 9(July).
9. Chen Y, Chang C, Chen H, Hsieh C, Tang K, Yang M, et al. Association between autophagy and inflammation in patients with rheumatoid arthritis receiving biologic therapy. Arthritis Res Ther 2018; 20(268): 1–11.
10. Ramiro S, Sepriano A, Chatzidionysiou K, Nam JL, Smolen JS van der HD, Al. E. Safety of synthetic and biological DMARDs: a systematic literature review informing the 2013 update of the EULAR recommendations for management of rheumatoid arthritis. Ann Rheum Dis 2014; 73(3): 529–35.
11. Li S, Liao W, Chen M, Shan S, Song Y, Zhang S, et al. Expression of Programmed Death-1 ( PD-1 ) on CD4 + and CD8 + T cells in Rheumatoid Arthritis. Inflammation 2014; 37(1): 116–21.
12. Mellado M, Laura Martinez-Munoz, Graciela Cascio, Pilar Lucas JP, Rodnguez-Frade and JM. T cell migration in rheumatoid. Front Immunol 2015; 6(July).
13. Kang R, Zeh HJ, Lotze MT, Tang D. The Beclin 1 network regulates autophagy and apoptosis. Cell Death Differ. Nature Publishing Group 2011; 18(4): 571–80.
14. Olmer M, Williams GW, Venkatanarayanan HB. Increased autophagy in CD4+T cells of rheumatoid arthritis patients results in T-cell hyperactivation and apoptosis resistance Jorg. Eur J Immunol 2016; 1–32.
15. Dai Y, Hu S. Recent insights into the role of autophagy in the pathogenesis of rheumatoid arthritis. Rheumatology (Oxford) 2016; 55(3): 403-10.
16. Guma M, Wang Y, Viollet B, Liu-bryan R. AMPK Activation by A-769662 Controls IL-6 Expression in Inflammatory Arthritis. Plose One 10(10) 2015; 1–13.
17. Thornton CC, Calay D, Birdsey GM, Bauer A, Mylroie H, Morley BJ, et al. Methotrexate-mediated activation of an AMPK- CREB-dependent pathway : a novel mechanism for vascular protection in chronic systemic in fl ammation. Basic Transl Res 2015; 1–10.
18. Kandemir FM, Kucukler S, Caglayan C, Gur C, Batil AA GI. Therapeutic effects of silymarin and naringin on methotrexate- induced nephrotoxicity in rats: Biochemical evaluation of anti-inflammatory, antiapoptotic, and antiautophagic properties. J Food Biochem 2017; 41(5).
19. Sanchez AM, Csibi A, Raibon A, Cornille K, Bernardi H, Candau R. AMPK Promotes Skeletal Muscle Autophagy Through Activation of Forkhead FoxO3a and Interaction With Ulk1. J Cell BioChem 2012; 113(2): 695-710.
20. Zheng F, Wu J, Zhao S, Luo Q, Tang Q, Yang L, et al. Baicalein increases the expression and reciprocal interplay of RUNX3 and FOXO3a through crosstalk of AMPK α and MEK / ERK1 / 2 signaling pathways in human non-small cell lung cancer cells. J Exp Clin Cancer Res 2015; 34: 41.
21. Xu K, Cai YS, Lu SM, Li XL, Liu L, Li Z et al. Autophagy induction contributes to the resistance to methotrexate treatment in rheumatoid arthritis fibroblast-like synovial cells through high mobility group box chromosomal protein 1. Arthritis Res Ther 2015; 17(374).
22. Beltrametti SP, Ianniello A, Ricci C. Chronotherapy with low-dose modified-release prednisone for the management of rheumatoid arthritis: a review. Ther Clin Risk Manag 2016; 12: 1763–76.
23. Smith LK, Cidlowski JA, Group ME. Glucocorticoid-induced apoptosis of healthy and malignant lymphocytes. Prog Brain Res 2016; 6123(10): 1–30.
24. Rockel JS, Kapoor M. Autophagy: controlling cell fate in rheumatic diseases. Nat Rev Rheumatol 2016; 12(9): 517–32.
25. Pattacini L, Boiardi L, Casali B SC. Differential effects of anti-TNFalpha drugs on fibroblast-like synoviocyte apoptosis. Rheumatology 2010; 49(3): 480–9.
26. Nikoletopoulou V, Palikaras KTN. Crosstalk between apoptosis, necrosis and autophagy. Biochim Biophys Acta 2013; 1833(12): 3448–59.
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Kardideh B, Sadeghalvad M, Samimi Z, Mohammadi Motlagh H R, Taghadosi M. Evaluation of Beclin-1 and Atg5 genes expression levels in peripheral blood cells of patients with rheumatoid arthritis . Feyz. 2019; 23 (2) :135-142
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