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:: Volume 5, Issue 3 (Quarterly 2001) ::
Feyz 2001, 5(3): 41-46 Back to browse issues page
Extraction and purification of the capsule of Staphylococcus Aureus type 2
Sayed asghar Havaie *, Taghi Naserpour Farivar, Mahmoud Saffari
Abstract:   (8761 Views)

History and Objectives: Extraction and purification of polysaccharide capsule is the first step for preparation preventive vaccines in infections as a result of encapsulated Staphylococcus aureus. This study was carried out to extract and purify Staphylococcus aureus type 2.

Materials and Methods: The descriptive and explorative strategy of this study was conducted on Staphylococcus aureus (Smith diffuse variant) in BHI broth in a shaking incubator (200 rpm) in an aerobic condition at a temperature of 37°C. The capsule was extracted using dilute acid and heating method. Then, dialysis was done overlight at 4°C and the product was lyophilized. Lyophilized powder was purified using a cellulose DEAE column (2.6×50 cm) by gradient method using bicarbonate ammonium buffers (0.01-1 M) at a velocity of 60 mt/h (Ion exchange chromatography). Finally absorption spectrum was plotted and purified capsule was analyzed using scanning spectrophotometer.

Results: Polysaccharide capsule was collected in final growth phase at 0.1 HCL and at a temperature of 100°C. Absorption spectrum were evaluated using H2SO4/galacial acetic acid assay at wavelength range of 350-620 nm. The result showed that chromatographs are well matched with previously purified antigens.

Conclusion and Recommendations: It is concluded that it is possible to extract and purify the capsule of Staphylococcus aureus type 2 and it is recommended to produce the related preventive vaccines.

Keywords: Staphylococcus aureus, Polysaccharide capsule, Final growth phase, Purified antigen, Extraction
Full-Text [PDF 1047 kb]   (1553 Downloads)    
Type of Study: Research | Subject: General
Received: 2008/11/10 | Published: 2001/10/15
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Havaie S A, Naserpour Farivar T, Saffari M. Extraction and purification of the capsule of Staphylococcus Aureus type 2 . Feyz. 2001; 5 (3) :41-46
URL: http://feyz.kaums.ac.ir/article-1-367-en.html

Volume 5, Issue 3 (Quarterly 2001) Back to browse issues page
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