:: Volume 22, Issue 6 (Bimonthly 2018) ::
Feyz 2018, 22(6): 573-580 Back to browse issues page
Evaluating the protective effect of 6 weeks resistance training and vitamin D intake on brain neuro-inflammatory factors in female rats with experimental autoimmune encephalomyelitis
Smatalsadat Mousavi , Ziya Fallahmohammadi * , Akbar Hajizadeh Moghaddam
Department of Exercise Physiology, Faculty of Sport Sciences, University of Mazandaran, I. R. Iran. , zia-falm@umz.ac.ir
Abstract:   (981 Views)
Background: Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease and is associated with reduced physical capacity. The aim of this study was to examine the protective effect of a 6-week resistance training program and vitamin D intake on the brain neuro-inflammatory factors in female Lewis rats with experimental autoimmune encephalomyelitis (EAE).
Materials and Methods: In this experimental study, four-week-old female Lewis rats (n=48) were randomly divided into 8 groups. Resistance training protocol was performed for 6 weeks and 5 days a week. A training program was started with a load equal to 50% of the body weight of the rat. Animals in the first two sessions performed 8 to 10 repetitions (climbing the ladder) at 2-minute breaks. The rats were immunized with the guinea pig's spit and complete adjuvant. The EAE model was induced at the end of the sixth week of the exercise. Rats received 5 μg of vitamin D3 in 150 μl of sesame oil per kilogram of their body weight via intraperitoneal injection once every two days for 2 weeks.
Results: The results showed that six weeks of resistance training with vitamin D had no significant effect on granulocyte-monocyte growth factor, nuclear transcription factor kB and transforming growth factor beta (P>0.05). However, Interleukin-17 values showed a significant difference in all three levels of training (P=0.000), supplements (P=0.045), and interactive training and supplementation (P=0.043).
Conclusion: The inadequacy of the training period (frequency and intensity of exercise) and the dose rate of vitamin D3 can be one of the possible causes of ineffectiveness of the present intervention.
Keywords: Resistance training, Neuro-inflammatory factors, Vitamin D, Multiple sclerosis
Full-Text [PDF 301 kb]   (238 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/08/1 | Accepted: 2018/11/21 | Published: 2019/01/30
1. Harlow DE, Honce JM, Miravalle AA. Re‌my‌el‌in‌ation therapy in multiple sclerosis. Front Neurol 2015; 6: 257-64.
2. Stromnes IM, Cerretti LM, Liggitt D, Harris RA, Goverman JM. Differential regulation of cen‌tral nervous system autoimmunity by TH1 and TH17 cells. Nat Med 2008; 14(3):337-42.
3. Tzartos JS, Friese MA, Craner MJ, Palace J, Newcombe J, Esiri MM, et al. Interleukin-17 production in central nervous system-infiltrating T cells and glial cells is associated with active disease in multiple sclerosis. Am J Pathol 2008; 172(1): 146-55.
4. Fischer HJ, Schweingruber N, Lühder F, Reic‌hardt HM. The potential role of T cell migration and chemotaxis as targets of glucocorticoids in multiple sclerosis and experimental autoimmune encephalomyelitis. Mol Cell Endocrinol 2013; 380(1): 99-107.
5. Souza PS, Gonçalves ED, Pedroso GS, Farias HR, Junqueira SC, Marcon R, et al. Physical exer‌cise attenuates experimental autoimmune enceph‌alo‌myelitis by inhibiting peripheral immune resp‌onse and blood-brain barrier disruption. Mol Neu‌robiol 2017; 54(6): 4723-37.
6. Moazzemi M, Fathi M, Soltani M, Gelradi N. The effects of aerobic exercise in water on cortisol levels and TGF-β in patients with multiple sclerosis. J Sabzevar Univ Med Sci 2014; 21(2):207-16.
7. Yan J, Greer JM. NF-κB, a potential therapeutic target for the treatment of multiple sclerosis. CNS Neurol Disord Drug Targets 2008; 7(6): 536-57.
8. El-Behi M, Ciric B, Dai H, Yan Y, Cullimore M, Safavi F, et al. The encephalitogenicity of TH17 cells is dependent on IL-1-and IL-23-induced production of the cytokine GM-CSF. Nat Immunol 2011; 12(6): 568-75.
9. Rasouli J, Ciric B, Imitola J, Gonnella P, Hwang D, Mahajan K, et al. Expression of GM-CSF in T cells is increased in multiple sclerosis and suppr‌e‌ssed by IFN-β therapy. J Immunol 2015; 194(11): 5085-93.
10. Kostic M, Stojanovic I, Marjanovic G, Ziv‌kovic N, Cvetanovic A. Deleterious versus prote‌ctive autoimmunity in multiple sclerosis. Cell Imm‌unol 2015; 296(2): 122-32.
11. Hirose L, Nosaka K, Newton M, Laveder A, Kano M, Peake J, Suzuki K. Changes in inflame‌matory mediators following eccentric exercise of the elbow flexors. Exerc Immunol Rev 2004; 10: 75-90.
12. Paulsen G, Benestad HB, StrØm-gundersen I, MØrkrid L, LappegÅrd KT, Raastad T. Delayed leukocytosis and cytokine response to high-force eccentric exercise. Med Sci Sports Exerc 2005; 37(11): 1877-83.
13. Bonsignore MR, Morici G, Santoro A, Pagano M, Cascio L, Bonanno A, et al. Circulating hemato‌poietic progenitor cells in runners. J Appl Physiol 2002; 93(5): 1691-7.
14. Alharbi FM. Update in vitamin D and multiple sclerosis. Neurosciences 2015; 20(4):329–35.
15. Mosayebi G, Ghazavi A, Salehi H. Effect of vita‌min D3 on leukocyte infiltration into the brain of C57/BL6 mice with experimental autoimmune encephalomyelitis. Yafte 2006; 8(3):85-91.
16. Van Etten E, Branisteanu DD, Overbergh L, Bouillon R, Verstuyf A, Mathieu C. Combination of a 1, 25-dihydroxyvitamin D 3 analog and a bispho‌sphonate prevents experimental autoimmune ence‌ph‌a‌lomyelitis and preserves bone. Bone 2003; 32(4): 397-404.
17. Cantorna MT, Humpal-Winter J, DeLuca HF. In vivo upregulation of interleukin-4 is one mech‌anism underlying the immunoregulatory effects of 1, 25-dihydroxyvitamin D3. Arch Biochem Bio‌ph‌ys 2000; 377(1): 135-8.
18. Tartibian B, Godrat-Garebagh Z, Gaeini A, Tol‌ouei-Azar J. Influence of 9-weeks aerobic exer‌cise and multivitamin supplement on inflam‌mation biomarkers as cardiovascular risk factor in non-athletic obese women. Zahedan J Res Med Sci 2013; 15(3): 30-5
19. Schleithoff SS, Zittermann A, Tenderich G, Berthold HK, Stehle P, Koerfer R. Vitamin D supple‌mentation improves cytokine profiles in pati‌ents with congestive heart failure: a double-blind, randomized, placebo-controlled trial. Am J Clin Nutr 2006; 83(4): 754-9.
20. Bherer L, Erickson KI, Liu-Ambrose T. A review of the effects of physical activity and exer‌cise on cognitive and brain functions in older adults. J Aging Res 2013; 657508.
21. DeBolt LS, McCubbin JA. The effects of home-based resistance exercise on balance, power, and mobility in adults with multiple sclerosis. Arch Phys Med Rehabil 2004; 85(2): 290-7.
22. Yaghmaei P Nabioni M, Mahdavi M, Meh‌r‌varz T, Nazari Z. The effect of different doses of bee venom serum levels of interleukin-6 in Lewis rats EAE (a model for studying multiple sclerosis). Pejouhandeh 2013; 18(2): 69-75.
23. Safarzadeh A, Hajizadeh Rostami M, Talebi Garakani E, Fathi R. The effect of progressive resis‌tance training on serum cocentrations of omentin-1 and lipid profile in male rats. J Sport Biosciences 2014; 6(3): 287-300.
24. Mosayebi G, Ghazavi A, Payani MA. The Effect of Vitamin D3 on the inhibition of experi‌mental autoimmune encephalomyelitis in C57BL/6 mice. Razi J Med Sci 2006; 13(52): 189-96.
25. Golzari Z, Shabkhiz F, Soudi S, Kordi MR, Hashemi SM. Combined exercise training reduces IFN-γ and IL-17 levels in the plasma and the supernatant of peripheral blood mononuclear cells in women with multiple sclerosis. Int Immuno‌pharmacol 2010; 10(11): 1415-9.
26. Hosseini S, Rakhshi N, Abdullah N, Jafari S, Nikravesh A. Vitamin D, the healthy and inflamed Immune System in multiple sclerosis. J North Kh‌o‌r‌asan Univ Med Sci 2014; 5(5):935-41.
27. Nieman DC, Konrad M, Henson DA, Kennerly K, Shanely RA, Wallner-Liebmann SJ. Variance in the acute inflammatory response to prolonged cycling is linked to exercise intensity. J Interferon Cytokine Res 2012; 32(1): 12-17.
28. Konrad M, Nieman DC, Henson DA, Kennerly KM, Jean F, Wallner-Liebmann SJ. The acute effect of ingesting a quercetin-based supplement on exer‌cise-induced inflammation and immune changes in runners. Int J Sport Nutr Exerc Metab 2011; 21(4): 338-46.
29. Suzuki K, Nakaji S, Yamada M, Liu Q, Kura‌kake S, Okamura N, et al. Impact of a competitive marathon race on systemic cytokine and neutrophil responses. Med Sci Sports Exerc 2003; 35(2): 348-55.
30. Zwesloot KA, John CS, Lawrence MM, Bat‌tista RA, Shanely RA. High-intensity interval trai‌ning ind‌uces a modest systemic inflammatory res‌ponse in active, young men. J Inflamm Res 2014; 9(7): 9–17.
31. Carrillo AE, Flynn MG, Pinkston C, Markofski MM, Jiang Y, Donkin SS, et al. Impact of Vitamin D supplementation during exercise training does not alter inflammatory biomarkers in overweight and obese subjects. Clin Nutr 2013; 32(3): 375-81
32. Mc Guire C, Prinz M, Beyaert R, Van Loo G. Nuclear factor kappa B (NF-κB) in multiple scl‌erosis pathology. Trends Mol Med 2013; 19(10): 604-13.

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