[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 22, Issue 6 (Bimonthly 2018) ::
Feyz 2018, 22(6): 555-563 Back to browse issues page
The effect of moderate intensity exercise on the activity of catalase enzyme and malondialdehyde in hippocampus area of diabetic male Wistar rats
Mohammad Rami * , Abdolhamid Habibi , Mojdeh Khajehlandi
Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, I. R. Iran. , m.rami@scu.ac.ir
Abstract:   (398 Views)
Background: Diabetes mellitus exacerbates oxidative stress and reduces the antioxidant defense system activity. As the brain has a high sensitivity to oxidative stress due to physiological and biochemical reasons and scientific evidence suggests the effect of regular exercise on reducing brain sensitivity to brain damage under pathophysiological conditions. Therefore, this study aimed at investigating the effect of moderate intensity exercise training on the activity of catalase enzyme and malondialdehyde in hippocampus area of diabetic male Wistar rats.
Materials and Methods: Twenty-four male rats (245±9.4 g) aged 10 weeks were divided into four groups (diabetic training, diabetic control, healthy training and healthy control). The rats of the diabetic group were diabetic by intraperitoneal injection of streptozotocin (STZ). The exercise program included 6 weeks of moderate intensity exercise. At the end of six weeks, the hippocampal tissue samples were extracted 24 hours after the last training session and the activity of catalase enzyme and malondialdehyde was evaluated.
Results: After the endurance training, the catalase levels in both diabetic training group (161.24±7.74) compared to the diabetic control group (148.55±8.05) and healthy training group (408.85±2.3) compared to the healthy control group (283.44±9.33) were significantly increased (P<0.05). Also, the level of malondialdehyde in the diabetic training group (9.65±1.75) was significantly decreased compared to the control diabetic group (13.23±1.01) (P>0.05).
Conclusion: It can be concluded that endurance training may be effective to increase the antioxidant role of catalase enzyme and reduce the amount of malondialdehyde in the hippocampus tissue of diabetic rats.   
Keywords: Exercise, Catalase, Malondialdehyde, Hippocampus
Full-Text [PDF 338 kb]   (158 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/07/4 | Accepted: 2018/10/27 | Published: 2019/01/30
1. Hosseini SA, Nezafat Absardi M, Shadmehri S, Salehi O, Hajisadeghi H. The Interactional Effects of Endurance Training and Aloe Vera Gel on Alanine Aminotransferase and Aspartate Aminotransferase levels in Diabetic Rats. Yafte 2018; 20(1):99-111. [in Persian]
2. Ghalavand A, Shakeryan S, Nikbakht A, Mehdi‌pour A, Monazamnezhad A, Delaramnasab M. Eff‌e‌c‌ts of aerobic training on cardiorespiratory factors in men with type 2 diabetes. J Diabetes Nursing 2014; 2(2): 8-17. [in Persian]
3. Robertson RP. Oxidative stress and impaired ins‌ulin secretion in type 2 diabetes. Curr Opin Pharm‌a‌col 2006; 6(6): 615-9.
4. Penckofer S, Schwertz D, Florczak K. Oxidative stress and cardiovascular disease in type 2 diabetes: the role of antioxidants and prooxidants. J Cardio‌vasc Nurs 2002; 16(2): 68–85.
5. Halliwell B. Free radicals and antioxidants: A per‌sonal view. Nutr Rev 1994; 52(8 Pt 1): 253-65.
6. Dixon IM, Kaneko M, Hata T, Panagia V, Dhalla NS. Alterations in cardiac membrane Ca2+ transport during oxidative stress. Mol Cell Biochem 1990; 99(2): 125-33.
7. Gupta M, Singal PK. Time course of structure, function and metabolic changes due to an exogenous source of oxygen metabolites in rat heart. Can J Physiol Phramacol 1989; 67(12): 1549-59.
8. Baynes JW. Perspective in diabetes. Role of oxidative stress in development of complication in diabetes. Diabetes 1991; 40 (4): 405-41.
9. [9] Kabel AM. Free radicals and antioxidants: role of enzymes and nutrition. World J Nutrition Health 2014; 2(3): 35-8.
10. Lobo V, Patil A, Phatak A, Chandra N. Free rad‌i‌cals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010; 4(8): 118-26.
11. Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 2002; 7(9):405-10.
12. Kirkman HN, Gaetani GF. Mamalian catalase: a venereable enzyme with new mysteries. Trends Biochem Sci 2007; 32(1): 44-50.
13. [13] Suh S, Jeong IK, Kim MY, Kim YS, Shin S, Kim SS, et al. Effects of resistance training and aer‌o‌bic exercise on insulin sensitivity in overweight Kor‌ean adolescents: a controlled randomized trial. Diab‌etes Metab J 2011; 35(4): 418-26.
14. Deng H, Wen Q, Luo Y, Huang Y, Huang R. Infl‌uence of different extracts from persimmon leaves on the antioxidant activity in diabetic mice. Zhong Nan Da Xue Xue Bao Yi Xue Ban 2012; 37(5): 469-73.
15. Vincent HK, Powers SK, Stewart DJ, Shanely RA, Demirel H, Naito H. Obesity is associated with increased myocardial oxidative stress. Int J Obes Relat Metab Disord 1999; 23(1): 67-74.
16. [16] Hovanloo F, Hedayati M, Abraham M, Abid Nazari H. The effect of endurance training in differ‌rent periods of time in the activities of antioxidant enzymes in rat liver. Med Res 2011; 35(1): 14-9. [in Persian]
17. Taheri B, Rezaeshirazi R. Effect of a six-week high-intensity interval training on antioxidant capa‌ci‌ty and lipid peroxidation in inactive men. Acta Med‌i‌ca 2016; 32: 1055-60. [in Persian]
18. Silva LA, Scheffer DL, Alves A, Pereira L T, Mon‌eretto DB, Tromm C. Effect of aerobic training of moderate and low volume on electron transport chain activity and oxidative stress markers in skel‌etal muscle. J Exerc Physiol Online 2015; 18(6): 81-93.
19. Toth C. Diabetes and neurodegeneration in the brain. Handb Clin Neurol 2014; 126: 489-511.
20. Sugimoto K, Rashid IB, Shoji M, Suda T, Yasujima M. Early changes in insulin receptor sig‌naling and pain sensation in streptozotocin-induced diabetic neuropathy in rats. JPain 2008; 9 (3): 237–45.
21. Adenowo AF, Ilori MF, Balogun FO, Kazeem MI. Protective effect of ethanol leaf extract of Carica papaya Linn (Caricaceae) in alloxan-induced diabetic rats. Tropical J Pharmaceutical Res 2014; 13(11): 1877-82.
22. Chae CH, Jung SL, An SH, Jung CK, Nam SN, Kim HT. Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating p-phosphatidylinositol 3-kinase acti‌vation in the soleus of diabetic rats, & quot. J Phy‌siol Biochem 2011; 67(2): 235-41.
23. Mohammad Kord, Investigating the effect of 6 weeks endurance activity on expression of DJ1 and CB2 proteins in hippocampus in male rats with diabetes. [Dissertation]. Lorestan. Lorestan Univ‌e‌r‌sity. 2018. [in Persian]
24. Luhova L, Lebeda A, Hedererova D, Pec P. Acti‌vities of amine oxidase, peroxidase and catalase in seedlings of Pisum sativum L. under different light conditions. Plant Soil Environ2003; 49(4): 151-7.
25. Kakkar P, Das B, Viswanathan PN, A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984; 21(2): 130-2.
26. Chen Z, He Y, Song C, Dong Z, Su Z, Xue J. Sericin can reduce hippocampal neuronal apoptosis by activating the Akt signal transduction pathway in a rat model of diabetes mellitus. Neural Regen Res 2012; 7(3): 197-201.
27. Ascensão A, Magalhães J, Soares J, Ferreira R, Neuparth M, Marques F, et al. Endurance training attenuates doxorubicin-induced cardiac oxidative damage in mice. Int J Cardiol 2005;100(3):451-60.
28. Nakanishi S, Suzuki G, Kusunoki Y, Yamane K, Egusa G, Kohno N. Increasing of oxidative stress from mitochondria in type 2 diabetic patients. Dia‌betes Metab Res Rev 2004; 20(5): 399-404.
29. Nojima H, Watanabe H, Yamane K, Kitahara Y, Sekikawa K, Yamamoto H, et al. Effect of aerobic exercise training on oxidative stress in patients with type 2 diabetes mellitus. Metabolism 2008; 57(2): 170–6.
30. Cunha TF, Bacurau AV, Moreira JB, Paixao NA, Campos JC, Ferreira JC, et al. Exercise training prevents oxidative stress and ubiquitinproteasome system overactivity and reverse skeletal muscle atro‌phy in heart failure. PLoS One 2012; 7(8): e41701.
31. Escribano BM, Tunez I, Requena F, Rubio MD, De Miguel R, Montilla P, et al. Effects of an aerobic training program on oxidative stress biomarkers in bulls. Veterin Med 2010; 55(9): 422–8.
32. Aksoy Y, Yapanoğlu T, Aksoy H, Demircan B, Öztaşan N, Canakci E, Malkoc I. Effects of endu‌r‌a‌nce training on antioxidant defense mechanisms and lipid peroxidation in testis of rats. Arch Androl 2006; 52(4): 319-23.
33. Finkel T, Holbrook NJ. Oxidants, oxidative st‌re‌ss and the biology of ageing. Nature 2000; 408 (6809): 239-47.
34. Alipour M, Salehi I, Ghadiri Soufi F. Effect of exercise on diabetes-induced oxidative stress in the rat hippocampus. Iran Red Crescent Med J 2012; 14(4): 222-8. [in Persian]
35. Atalay M, Sen CK. Physical Exercise and Anti‌oxidant Defenses in the Hearta. Ann N Y Acad Sci 1999; 874(1): 169-77.‌
36. Ji LL, Radak Z, Goto S. Hormesis and exercise: how the cell copes with oxidative stress. Am J Phar‌macol Toxicol 2008; 3(1): 41-55.
37. Valadoa A, Tavaresb PC, Pereirac L, Ribeiroa CF. Anaerobic exercise and oxidative stress effect of the intense exercise on nitric oxide and malon‌di‌aldehyde. Int Conference on Cellular & Molecular Biology-Biophysics & Bioengineering. Greece 2007; 26-8.
38. Gupt AM, Kumar M, Sharma RK, Misra R, Gup A. Effect of moderate aerobic exercise training on pulmonary functions and Its correlation with the antioxidant status. National J Med Res 2015; 5(2): 136-9.
39. Songstad NT, Kaspersen KH, Hafstad AD, Bas‌net P, Ytrehus K, Acharya G. Effects of High Intensity Interval Training on Pregnant Rats, and the Placenta, Heart and Liver of Their Fetuses. PLoS One 2015; 10(11): e0143095.
40. Acikgoz O, Aksu I, Topcu A, Kayatekin BM. Acu‌te exhaustive exercise does not alter lipid peroxidation levels and antioxidant enzyme activities in rat hippocampus, prefrontal cortex and striatum. Neurosci Lett 2006; 406(1): 148-51.
41. Fusco D, Colloca G, Lo Monaco MR,Cesari M. Effects of antioxidant supplementation on the aging process. Clin Interv Aging 2007; 2(3): 377–87.
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rami M, Habibi A, Khajehlandi M. The effect of moderate intensity exercise on the activity of catalase enzyme and malondialdehyde in hippocampus area of diabetic male Wistar rats. Feyz. 2018; 22 (6) :555-563
URL: http://feyz.kaums.ac.ir/article-1-3633-en.html

Volume 22, Issue 6 (Bimonthly 2018) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3921