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:: Volume 22, Issue 2 (Bimonthly 2018) ::
Feyz 2018, 22(2): 206-213 Back to browse issues page
Study of drug resistance of Staphylococcus aurous and Pseudomonas aeruginosa strains isolated from environmental samples of Hamadan educational hospitals in 2017 using disk diffusion and minimum inhibitory concentration
Masoume Einabadi , Fahimeh Abdolerahmani , Rasoul Yousefi Mashoof , Hossein Vazini , Ali Shakerimoghaddam , Azad Khaledi , Ahmad Piroozmand , Pezhman Karami *
Department of Microbiology, Faculty of Medicine, Medical Sciences University, Hamadan, I. R. Iran. , pezhmankarami@gmail.com
Abstract:   (355 Views)
Background: Staphylococcus aureus and Pseudomonas aeruginosa are the most important bacteria causing the nosocomial infections, which are resistant to most of the antibiotics. The aim of this study was to evaluate the drug resistance of S. aureus and P. aeruginosa strains isolated from environmental samples of Hamedan educational hospitals using disk diffusion and minimum inhibitory concentration (MIC) methods.
Materials and Methods: In this descriptive cross-sectional study, 400 samples were collected from Hamedan educational hospitals. To assess the antibiotic susceptibility of 10 common antibiotics, the agar dilution (Kirby-Bauer) method was used. Also, to determine the MIC of S. aureus and P. aeruginosa, vancomycin and ciprofloxacin antibiotics were used.
Results: From a total of 400 samples, 39 (9.7%) isolates were P. aeruginosa and 28 (7%) were S. aureus. Staphylococcus aureus showed the highest resistance to ofloxacin (82.1%) and the highest drug resistance to P. aeruginosa was related to meropenem (82%). Also, the highest MIC and maximum bactericidal concentration (MBC) for S. aureus to vancomycin were 128 and 256, respectively. In P. aeruginosa, the highest MIC and MBC to ciprofloxacin were 128 and 256, respectively.
Conclusion: Staphylococcus aureus and P. aeruginosa showed the highest resistance to ofloxacin and meropenem, respectively. Considering the rapid increase of antibiotic resistance, accurate evaluation of the antibiotic resistance pattern of the bacteria is required.
Keywords: Antibiotic resistance, Staphylococcus aureus, Pseudomonas aeruginosa
Full-Text [PDF 246 kb]   (45 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2017/12/23 | Accepted: 2018/02/17 | Published: 2018/02/17
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Einabadi M, Abdolerahmani F, Yousefi Mashoof R, Vazini H, shakerimoghaddam A, Khaledi A, et al . Study of drug resistance of Staphylococcus aurous and Pseudomonas aeruginosa strains isolated from environmental samples of Hamadan educational hospitals in 2017 using disk diffusion and minimum inhibitory concentration . Feyz. 2018; 22 (2) :206-213
URL: http://feyz.kaums.ac.ir/article-1-3496-en.html


Volume 22, Issue 2 (Bimonthly 2018) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
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