[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 21, Issue 6 (Bimonthly 2017) ::
Feyz 2017, 21(6): 577-583 Back to browse issues page
Identification of Mycobacterium species isolated from patients using high-performance liquid chromatography in Tehran during 2014-2015
Aliyar Mirzapour , Masoud Yousefi , Saeed Zaker-Bostanabadi , Abdolrazagh Hashemi-Shahraki , Ali Nazari-Alam *, Soltan Ahmad Ebrahimi
Department of Microbiology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I. R. Iran. , nazarialam.ali@gmail.com
Abstract:   (321 Views)
Background: Non-tuberculous mycobacteria (NTM) are defined as mycobacteria other than Mycobacterium tuberculosis (MTB), which do not cause tuberculosis or leprosy. Early and precise diagnosis of NTM is particularly important for the correct epidemiological control and specific treatments. The aim of this study was to identify the mycobacterium species isolated from patients referred to hospitals in Tehran using the high-performance liquid chromatography (HPLC) method.
Materials and Methods: In this cross-sectional descriptive study, a collection of isolates (n=20) was obtained from clinical specimens submitted to the Masoud Laboratory in Tehran, Iran, during 2014-2015. The strains were isolated from sputum, urine, blood, and various sterile body fluid specimens. Chromatography was conducted at a flow rate with a curvilinear gradient of methanol and methylene chloride, beginning at 98% methanol containing 2% methylene chloride and ending at 35% methanol contained in 65% methylene chloride.
Results: From a total of 20 clinical isolates, 8 isolates (40%) were identified as Mycobacterium abscessus, 6 isolates (30%) M. tuberculosis, 3 isolates (15%) M. intracellulare and 3 isolates (15%) M. fortuitum.
Conclusion: For the proper treatment, rapid differentiation between MTB and NTM should be performed in persons who are diagnosed with or are suspected of having infectious TB disease. So, the HPLC method can be suggested as a cost-effective, specific and reliable method for rapid identification of MTB and differentiation of NTM strain from positive cultures isolated from clinical specimens.
Keywords: Mycobacterium tuberculosis, Chromatography high pressure liquid, Tuberculosis, Sputum, Identification
Full-Text [PDF 274 kb]   (55 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/05/25 | Accepted: 2017/12/12 | Published: 2017/12/13
1. Adigun R, Bhimji S. Tuberculosis. StatPearls 2017. Available at: https://www.ncbi.nlm.nih.gov/books/NBK441916.
2. Ahmed I, Jabeen K, Hasan R. Identification of non-tuberculous mycobacteria isolated from clinical specimens at a tertiary care hospital: a cross-sectional study. BMC Infect Dis 2013; 13(1): 493.
3. Lai CC, Tan CK, Chou CH, Hsu HL, Liao CH, Huang YT, et al. Increasing Incidence of Nontuberculous Mycobacteria, Taiwan, 2000–2008. Emerg Infect Dis 2010; 16(2): 294-6.
4. Rezntkov M, Robinson E. Serologically identical Battey mycobacteria from sputa of healthy piggery workers and lesions of pigs. Aust Vet J 1970; 46(12): 606-7.
5. Nasiri MJ, Dabiri H, Darban-Sarokhalil D, Hashemi Shahraki A. Prevalence of Non-Tuberculosis Mycobacterial Infections among Tuberculosis Suspects in Iran: Systematic Review and Meta-AnalysisPLoSone 2015; 10(6): e0129073
6. Moore JE, Kruijshaar ME, Ormerod LP, Drobniewski F, Abubakar I. Increasing reports of non-tuberculous mycobacteria in England, Wales and Northern Ireland, 1995-2006. BMC Infect Dis 2010; 10: 612.
7. Henry MT, Inamdar L, O'Riordain D, Schweiger M, Watson JP. Nontuberculous mycobacteria in non‐HIV patients: epidemiology, treatment and response. Eur Respir J 2004; 23(5): 741-6.
8. Piersimoni C, Scarparo C. Pulmonary infections associated with non-tuberculous mycobacteria in immunocompetent patients. Lancet Infect Dis 2008; 8(5): 323-34.
9. Tabarsi P, Baghaei P, Farnia P, Mansouri N, Chitsaz E, Sheikholeslam F, et al. Nontuberculous mycobacteria among patients who are suspected for multidrug-resistant tuberculosis—need for earlier identification of nontuberculosis mycobacteria. Am J Med Sci 2009; 337(3): 182-4.
10. Shojaei H, Heidarieh P, Hashemi A, Feizabadi MM, Naser AD. Species identification of neglected nontuberculous mycobacteria in a developing country. Jpn J Infect Dis 2011; 64(4): 265-71.
11. Khosravi Boroujeni AD, Hashemi Shahraki A, Hashemzadeh M, Sheini Mehrabzadeh R. Prevalence of nontuberculous mycobacteria in hospital watersof major cities of Khuzestan province, Iran. Eur Respir J 2016; 48( 60).
12. Ding LW, Lai CC, Lee LN, Hsueh PR. Disease caused by non-tuberculous mycobacteria in a university hospital in Taiwan, 1997-2003. Epidemiol Infect 2006; 134(5): 1060-7.
13. Lai CC, Lee LN, Ding LW, Yu CJ, Hsueh PR, Yang PC. Emergence of disseminated infections due to nontuberculous mycobacteria in non-HIV-infected patients, including immunocompetent and immunocompromised patients in a university hospital in Taiwan. J Infect 2006; 53(2): 77-84.
14. Wagner D, van Ingen J, Adjemian J, Lange C, Prevots DR, Griffith D, et al. Annual prevalence and treatment estimates of nontuberculous mycobacterial pulmonary disease in Europe: A NTM-NET collaborative study. Eur Respir J 2014; 44(Suppl58): P1067.
15. Junior CAC, Torre CAL, Souza Figueiredo EE, Lilenbaum W, Paschoalin VMF. Application of High Performance Liquid Chromatography for Identification of Mycobacterium spp. Tub Expand Knw 2015; 1: 152.
16. Furlanetto LV, Conte-Júnior CA, De Souza Figue EE, Duarte RS, Lilenbaum W, Silva JT, et al. HPLC Protocol for Identification of Mycobacterium spp. from Clinical Samples of Human and Veterinary J Microbiol Res 2014; 4(6): 193-200.
17. Mirzapour A, Alam AN, Bostanabadi SZ, Shahraki AH, Ebrahimi SA, Yousefi M. Identification of nontuberculous mycobacteria by high-performance liquid chromatography from patients in tehran. Int J Mycobacteriol 2016; 5 Suppl 1: S214.
18. Subedi S, Kong F, Jelfs P, Gray TJ, Xiao M, Sintchenko V, et al. 16S-23S Internal Transcribed Spacer Region PCR and Sequencer-Based Capillary Gel Electrophoresis has Potential as an Alternative to High Performance Liquid Chromatography for Identification of Slowly Growing Nontuberculous Mycobacteria. PLoS one 2016; 11(10): e0164138.
19. Murray SJ, Barrett A, Magee JG, Freeman R. Optimisation of acid fast smears for the direct detection of mycobacteria in clinical samples. J Clin Pathol 2003; 56(8): 613-5.
20. Butler WR, Jost KC Jr, Kilburn JO. Identification of mycobacteria by high-performance liquid chromatography. J Clin Microbiol 1991; 29(11): 2468-72.
21. Jeong J, Kim SR, Lee SH, Lim JH, Choi JI, Park JS, et al. The use of high performance liquid chromatography to speciate and characterize the epidemiology of mycobacteria. Lab Med 2011; 42(10): 612-7.
22. Lawn SD, Mwaba P, Bates M, Piatek A, Alexander H, Marais BJ, et al. Advances in tuberculosis diagnostics: the Xpert MTB/RIF assay and future prospects for a point-of-care test. Lancet Infect Dis 2013; 13(4): 349-61.
23. Jang MA, Koh WJ, Huh HJ, Kim SY, Jeon K, Ki CS, et al. Distribution of nontuberculous mycobacteria by multigene sequence-based typing and clinical significance of isolated strains. J Clin Microbiol 2014; 52(4): 1207-12.
24. Hsueh PR, Liu YC, So J, Liu CY, Yang PC, Luh KT. Mycobacterium tuberculosis in Taiwan. J Infect 2006; 52(2): 77-85.
25. Namkoong H, Kurashima A, Morimoto K, Hoshino Y, Hasegawa N, Ato M, et al. Epidemiology of pulmonary nontuberculous mycobacterial disease, Japan. Emerg Infect Dis 2016; 22(6): 1116-8.
26. Adjemian J, Frankland TB, Daida YG, Honda JR, Olivier KN, Zelazny A, et al. Epidemiology of Nontuberculous Mycobacterial Lung Disease and Tuberculosis, Hawaii, USA. Emerg Infect Dis 2017; 23(3): 439-47.
27. Kendall BA, Winthrop KL. Update on the Epidemiology of Pulmonary Nontuberculous Mycobacterial Infections. Semin Respir Crit Care Med 2013; 34(1): 87-94.
28. Prevots DR, Marras TK. Epidemiology of human pulmonary infection with nontuberculous mycobacteria: a review. Clin Chest Med 2015; 36(1): 13-34.
29. Zilaee M, Firozeh F, Moniri R, Sehat M, Bidgoli Z. Prevalence of non-tuberculous mycobacteria isolated from patients referring to tuberculosis center of Kashan University of Medical Sciences. SJKUMS 2016; 21(5): 50-9.
30. Burman WJ, Reves RR. Review of false-positive cultures for Mycobacterium tuberculosisand recommendations for avoiding unnecessary treatment. Clin Infect Dis 2000; 31(6): 1390-95.
31. Jeong J, Kim SR, Chang CL, Lee SH. Distribution and Clinical Significance of Nontuberculous Mycobacteria Identified by High Performance Liquid Chromatography in Clinical Specimens. Korean J Clin 2008; 11(1): 34-42.
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirzapour A, Yousefi M, Zaker-Bostanabadi S, Hashemi-Shahraki A, Nazari-Alam A, Ebrahimi S A. Identification of Mycobacterium species isolated from patients using high-performance liquid chromatography in Tehran during 2014-2015. Feyz. 2017; 21 (6) :577-583
URL: http://feyz.kaums.ac.ir/article-1-3349-en.html

Volume 21, Issue 6 (Bimonthly 2017) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.052 seconds with 843 queries by yektaweb 3600