:: Volume 21, Issue 1 (Bimonthly 2017) ::
Feyz Med Sci J 2017, 21(1): 35-41 Back to browse issues page
Association of Interleukin-27 gene rs153109 polymorphism and chronic hepatitis B infection
Samira Mokhtari , Seyyed Masud Hosseini , Seyyed Reza Mohebbi * , Pedram Azimzadeh , Mohammad Reza Zali
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, I. R. Iran. , sr.mohebbi@sbmu.ac.ir
Abstract:   (5008 Views)

Background: According to World Health Organization (WHO) report about 400 million people are chronically infected with hepatitis B virus (HBV). Host immune responses which are mainly controlled by cytokines, can be either effective in disease progression or control the infection. Interleukin-27 (IL-27) is a pro-inflammatory cytokine which promotes Th1 responses. Genetic variations (e.g. single nucleotide polymorphisms [SNPs]) can affect the product or activity of IL-27 gene. The aim of present study was to determine the association between IL-27 rs153109 and chronic HBV infection among the Iranian population.

Materials and Methods: In this study chronic HBV patients (n=120, Anti-HBc Ab positive and HBsAg positive for more than 6 months) and controls (n=120) from healthy individuals referred to Tehran Taleghani hospital (2013-2014) were studied. Genotypes of IL-27 gene polymorphism were detected by PCR-RFLP. DNA sequencing was applied on 10% of samples to validate the genotyping results. The studied variables were polymorphism genotypes/alleles, clinical status, age and gender.

Results: Results showed no statistically significant difference for patients and control groups   neither in genotype frequencies of AA among the chronic group (30%) compared to healthy controls (32.5%) (P=0.368); nor in allele frequency A) 60.4%) for patients against A 59.2% in control groups (P=0.780).

Conclusion: Despite the importance of IL-27 in the immune response, the findings of this study suggests that genetic variants of IL-27 SNP 153109A/G were not associated with susceptibility to the chronic infection of HBV.
Keywords: Chronic HBV infection, IL-27, SNP, rs153109
Full-Text [PDF 265 kb]   (1327 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/02/27 | Revised: 2017/07/19 | Accepted: 2017/02/27 | Published: 2017/02/27
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