[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 11, Issue 2 (Quarterly 2007) ::
Feyz 2007, 11(2): 6-11 Back to browse issues page
Effect of vitrification with single agent in stepwise manner using different concentrations of ethylene gycol on the morphology of mouse ovarian tissue
Elaheh Mirrasekhian , Mogdeh Salehnia
Tarbiat Modarres University of Medical Sciences , mogdeh@dr.com
Abstract:   (17534 Views)

Background: Considering the importance of cryoprotection and dehydration manner in the vitrification procedure, the aim of this study was to evaluate the effect of this technique using different concentrations of ethylene glycol (EG) as a single agent in stepwise manner and different dehydration time on the mouse ovarian tissue.

Materials and Methods: Thirty five NMRI adult mice (6-10 weeks) were sacrificed and their ovarian tissues were divided into control, toxicity test and vitrified groups. After dehydration of samples in increasing concentration of EG (2 and 4 M) for 1 min in every group the vitrification procedure was done using final concentration of 6 or 8 M of EG in different times (4,6, 8 min). The ovarian tissue in each vitrified group was stored in liquid nitrogen for one week. After warming, the samples were washed in 1 M sucrose solution. The samples of fresh, vitrified and toxicity test groups were fixed in 10% formaldehyde solution and embedded in paraffin. Then serial sections were prepared, stained with hematoxylen and eosin and studied morphologically.

Results: The morphology of ovarian tissue in all groups with different concentrations of EG and different dehydration times was almost the same. The preantral and antral follicles were well preserved especially on the cortical part of ovary they had a normal morphology.

Conclusion: The results showed that high concentration of EG (6 or 8 M) was not toxic and dehydration time from 4 to 8 minutes had no harmful effect on the structure of ovarian tissue during vitrification and warming. However, more additional investigations are recommended.

Keywords: Ethylene glycol, Ovarian tissue, Stepwise manner vitrification
Full-Text [PDF 210 kb]   (1764 Downloads)    
Type of Study: Research | Subject: General
Received: 2008/10/26 | Published: 2007/07/15
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirrasekhian E, Salehnia M. Effect of vitrification with single agent in stepwise manner using different concentrations of ethylene gycol on the morphology of mouse ovarian tissue. Feyz 2007; 11 (2) :6-11
URL: http://feyz.kaums.ac.ir/article-1-32-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 11, Issue 2 (Quarterly 2007) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.05 seconds with 46 queries by YEKTAWEB 4645