[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 20, Issue 5 (Bimonthly 2016) ::
Feyz 2016, 20(5): 454-460 Back to browse issues page
The presence of Helicobacter pylori in gallbladder mucosa of patients with chronic cholecystitis
Farzad Kheirmand *, Fahimeh Valadi, Kamran Dolatshahi
School of Medicine, Najafabad Branch, Islamic Azad University, Najafabad, I. R. Iran. , farzadkheirmand@yahoo.com
Abstract:   (1835 Views)

Background: Helicobacter pylori (H. pylori) has infected over half of the world’s population. Among the long-term outcomes of H.pylori infection is gastric malignancies, particularly gastric cancer and gastric mucosa-associated tissue (MALT) lymphoma. In recent years, the attention has been drawn to the possible association of H. pylori infections not only with gastric and duodenal disorders but also with several gastrointestinal diseases (e.g. liver and biliary diseases). H. pylori have recently found to be associated with some diseases of the biliary tree. Earlier reports on the detection of H. pylori in the gallbladder mucosa of patients with biliary disease have shown contradictory results which requires further studies. The aim of this cross-sectional study was to determine the presence of H. pylori in gallbladder mucosa of patients with a diagnosis of chronic cholecystitis using histological study of surgical gallbladder specimens.

Materials and Methods: In the present study, gallbladder samples (total no=70) from patients with chronic cholecystitis were collected at Shariati hospital (Isfahan, Iran) from February to April 2015. Giemsa staining was used to detect H. pylori in gallbladder mucosa.

Results: Using Giemsa staining in 18 (25.7%) out of 70 patients gallbladder mucosa were positive for H. pylori. There was an association between the presence of H. pylori in the gallbladder mucosa and chronic cholecystitis. (P<0.001)

Conclusion: H. pylori can be detected in gallbladder mucosa of patients with chronic cholecystitis. Further studies are needed to support these findings.

Keywords: Helicobacter pylori, Gallbladder tissue, Chronic cholecystitis
Full-Text [PDF 351 kb]   (674 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/11/13 | Accepted: 2016/11/13 | Published: 2016/11/13
References
1. Peura DA, Crowe SE. Helicobacter pylori. In: Feldman M, Friedman LS, Brandt LJ, editors. Sleisenger and Fordtran’s gastrointestinal and liver disease: pathophysiology, diagnosis, management. 9th ed. Canada: Saunders an important of Elsevier Inc; 2010. p. 833-43.
2. Abdul-nabi HM, Elkatib WA, Al-khafaji AJN, Abdulameer E. Gallbladder Colonization by H. Pylori in Patients with Symptomatic Gallstones Disease. Med J Babylon 2013; 10(3): 761-69.
3. Mirbagheri SA, Khajavirad N, Rakhshani N, Ostovaneh MR, Eshagh Hoseini SM, Hoseini V. Impact of Helicobacter pylori Infection and Microscopic Duodenal Histopathological Changes on Clinical Symptoms of Patients with Functional Dyspepsia. Dig Dis Sci 2012; 57(4): 967-72.
4. Yucebilgili K, Mehmetoglu T, Gucin Z, Salih BA. Helicobacter pylori DNA in gallbladder tissue of patients with cholelithiasis and cholecystitis. J Infect Dev Ctries 2009; 3(11): 856-9.
5. Fikry AA, Kassem AA, Shahin D, Shabana HAS, Mostafa S. Helicobacter pylori infection in patients with chronic calcular cholecystitis a cross–sectional study. J Surgery 2014; 2(4): 58-62.
6. Takahashi Y, Yamamichi N, Shimamoto T, Mochizuki S, Fujishiro M, Takeuchi C, et al. Helicobacter pylori infection is positively associated with gallstones: a large-scale cross-sectional study in Japan. J Gastroenterol 2014; 49(5): 882-9.
7. Neri V, Margiotta M, Francesco VD, Ambrosi A, Valle ND, Fersini A, et al. DNA sequences and proteic antigens of H. pylori in cholecystic bile and tissue of patients with gallstones. Aliment Pharmacol Ther 2005; 22(8): 715-20.
8. Fukuda K, Kuroki T, Tajima Y, Tsuneoka N, Kitajima T, Matsuzaki S, et al Comparative analysis of Helicobacter DNAs and biliary pathology in patients with and without hepatobiliary cancer. Carcinogenesis 2002; 23(11): 1927-31.
9. Villegas L, Pappas TN. Operative Management of Cholecystitis and Cholelithiasis. In: Yeo CJ, editor. Shackelford’s Surgery of the Alimentary Tract. 7th ed. China: Elsevier Saunders; 2013. p. 1315-25.
10. Khalaji N, Abdullahzade N, Poourghassem J, Maleki M. The relationship between age, sex and location of the patients and the outbreak of acute and chronic cholecystitis in patients operated in Imam Khomeini hospital of Urimia. J Urimia Nurs Midwfery Fac 2014; 11(11): 884-91. [in Persian]
11. Crawford JM, Liu C. Liver and Biliary Tract. In: Kumar V. Robbins and Cotran pathologic basis of disease. 8th ed. China: Saunders Elsevier; 2010. p. 833-90.
12. Karagin PH, Stenram U, Wadstrom T, Ljungh A. Helicobacter species and common gut bacterial DNA in gallbladder with cholecystitis. World J Gastroenterol 2010; 16(38): 4817-22.
13. Jahani Sherafat S, Tajeddin E, Reza Seyyed Majidi MR, Vaziri F, Alebouyeh M, Mohammad Alizadeh AH, et al. Lack of Association between Helicobacter pylori Infection and Biliary Tract Diseases. Polish J Microbiol 2012; 61(4): 319-22.
14. Sabbaghian MS, Ranaudo J, Zeng L, Alongi AP, Perez-Perez G, Shamamian P. Identification of Helicobacter spp. In bile and gallbladder tissue of patients with symptomatic gallbladder disease. HPB (Oxford) 2010; 12(2): 129-33.
15. Moricz Ad, Melo M, Castro AM, Campos TD, Silva RA, Pacheco AM Jr. Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder. Acta Cir Bras 2012; 25(3): 218-24.
16. Arismendi-Morillo G, Cardozo-Ramones V, Torres-Nava G, Romero-Amaro Z. Histopatho‌logical study of the presence of Helicobacter pylori-type bacteria in surgical specimens from patients with chronic cholecystitis. Gastroenterol Hepatol 2011; 34(7): 449-53.
17. Hashemi F, Alamdar S. Correlation between Helicobacter pylori and Chronic Cholecystitis in Gallbladder Specimens. Razi J Med Sci 2002; 9(31): 623-8. [in Persian]
18. Bahrami AR, Rahimi E, Safaei HG. Detection of Helicobacter pylori in City Water, Dental Units’ Water, and Bottled Mineral Water in Isfahan, Iran. ScientificWorldJournal 2013; 2013: 280510.
19. Hosseini E, Poursina F, Van de wiele T, Safaei HG, Adibi P. Helicobacter pylori in Iran: A systematic review on the association of genotypes and gastroduodenal diseases. J Res Med Sci 2012; 17(3): 280–92.
20. Lee JY, Kim N. Diagnosis of Helicobacter pylori by invasive test: histology. Ann Transl Med 2015; 3(1): 10.
21. Boldt MS, Pereira RD, Barbosa JA. Histological identification of H.pylori stained by hematoxylin-eosin and Giemsa: review for quality control. J Bras Patol Med Lab 2015; 51(2): 108-12.
22. Pandya HB, Patel JS, Agravat HH, Patel SB, Thakkar MC. Identification of Helicobacter pylori by different conventional staining techniques and its comparison with polymerase chain reaction. Saudi Med J 2013; 34(9): 942-8.
23. Boomkens SY, de Rave S, Pot RG, Egberink HF, Penning LC, Rothuizen J, et al. The role of Helicobacter spp. In the pathogenesis of primary biliary cirrhosis and primary sclerosing cholangitis. FEMS Immunol Med Microbiol 2005; 44(2): 221-5.
24. Krasinskas AM, Yao A, Randhawa P, Dore MP, Sepulveda AR. Helicobacter pylori May Play a Contributory Role in the Pathogenesis of Primary Sclerosing Cholangitis. Dig Dis Sci 2007; 52(9): 2265-70.
25. Leone N, Pellicano R, Brunello F, Cutufia MA, Berrutti M, Fagoonee S, et al. Helicobacter pylori seroprevalence in patients with cirrhosis of the liver and hepatocellular carcinoma. Cancer Detect Prev 2003; 27(6): 494-7.
26. Takahashi Y, Yamamichi N, Shimamoto T, Mochizuki S, Fujishiro M, Takeuchi C, et al. Helicobacter pylori infection is positively associated with gallstones: a large-scale cross-sectional study in Japan. J Gastroenterol 2014; 49(5): 882-9.
27. Bulajic M, Maisonneuve P, Schneider-Brachert W, Muller P, Reischl U, Stimec B, et al. Helicobacter pylori and the Risk of Benign and Malignant Biliary Tract Disease. Cancer 2002; 95(9): 1946-53.
28. Xu X, Sun Q, Liu Z, Zhang L, Luo Z, Xia Y, et al. The correlation between the malignant proliferation on cholangiocarcinoma cell lines and in bile from Helicobacter pylori infected patients with biliary tract stones. J Toxicol Environ Health Sci 2010; 2(2): 17-23.
29. Smoot DT, Elliott TB, Verspaget HW, Jones D, Allen CR, Vernon KG, et al. Influence of Helicobacter pylori on reactive oxygen-induced gastric epithelial cell injury. Carcinogenesis 2000; 21(11): 2091-5.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Kheirmand F, Valadi F, Dolatshahi K. The presence of Helicobacter pylori in gallbladder mucosa of patients with chronic cholecystitis. Feyz. 2016; 20 (5) :454-460
URL: http://feyz.kaums.ac.ir/article-1-3180-en.html


Volume 20, Issue 5 (Bimonthly 2016) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3925