:: Volume 21, Issue 5 (Bimonthly 2017) ::
Feyz 2017, 21(5): 443-449 Back to browse issues page
Study of antibiotic resistance pattern and incidence of pathogenic genes of mgtC, spi4R, agfA, invE/A and ttrC in Salmonella infantis isolated from clinical specimens
Roya Aghdasi-Araghinezhad, Kumarss Amini *
Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, I. R. Iran. , dr_kumarss_amini@yahoo.com
Abstract:   (2086 Views)
Background: The importance of the health of red meat, poultry and eggs in human nutrition is very high. One of the factors that jeopardize the health of poultry food products is the bacterial family of Enterobacteriaceae, especially Salmonella. The aim of this study was to detect pathogenic genes in Salmonella infectious bacteria isolated from stool specimens using the multiple PCR assay.
Materials and Methods: Selective and specific media for isolation of Salmonella were used. Primary isolation was carried out using Peptone water, Rapaport, selenite cysteine, MacConky agar and xylose-lysine deoxycholate agar. To confirm the diagnosis, biochemical tests including TSI, urea, endodontic, and citrate were used. The Salmonella Polyvalent Kit was used to determine Salmonella groups and mgtC, spi4R, agfA, invE/A and ttrC genes were studied in 60 samples by the multiple PCR method.
Results: The results showed that all samples had 2 genes mgtC and ttrC, and none of the samples showed resistance to cefepime. Of the 60 samples of Salmonella, none were resistant to cefepime and ceftriaxone; 38.8% of the samples were resistant to amoxicillin, 53% to erythromycin and 38.3% to sulfamethoxazole.
Conclusion: It can be concluded that cefepime is the best selective drug for the treatment of Salmonella infections. Identification and validation of genes in the region's bacteria can play a role in the broad epidemiological examination, antibiotic resistance, vaccine production, level of virulence, prevention and treatment. Also, evaluation of these genes in the samples for their virulence index is very important.
Keywords: Salmonella infantis, Pathogenic genes, Antibiotic resistance, Multiple PCR
Full-Text [PDF 288 kb]   (821 Downloads)    
Type of Study: Research | Subject: General
Received: 2016/11/13 | Accepted: 2017/05/29 | Published: 2017/11/15
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