:: Volume 20, Issue 5 (Bimonthly 2016) ::
Feyz 2016, 20(5): 441-446 Back to browse issues page
Association of miR-143 rs41291957 and rs4705342 genetic variants with endometriosis risk in infertile women
Elaheh Nimi-Hoveidi, Leila Kohan *, Seyyedeh Sara Hashemi
Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, I. R. Iran. , kohan@iaua.ac.ir
Abstract:   (2438 Views)

Background: Infertility is one of the major human problems. One of the most important causes of infertility is endometriosis. Endometriosis, defined as the growth of endometrial tissue outside the uterine cavity, is a common gynecological disorder. Recent research has shown that miRNAs and their target mRNAs are differentially expressed in endometriosis. The aim of this study was to investigate the association of miR-143 rs41291957 and rs4705342 gene variants with endometriosis in infertile women.

Materials and Methods: This case-control study was done on Iranian women (n=303) from March to February 2014. Blood samples from infertile women (n=77) with endometriosis and age-matched healthy women (n=226) at the Zeinabieh Hospital (Shiraz, Iran) were collected. The MiR-143 rs4705342 and rs41291657 genotypes were determined using Tetra-ARMS PCR and PCR-RFLP methods, respectively.

Results: There was a significant difference in the genotype distribution and allele frequency of rs4705342 polymorphism between case and control groups; TC genotype (OR: 2.3, 95%CI: 1-5.3, P=0.04) and C allele were associated with an increased risk of endometriosis (OR: 2.24, 95% CI: 1-4.9, P=0.04). Also, an allele of rs41291957 polymorphism showed a significant association with susceptibility to endometriosis (OR: 2, 95% CI: 1-4, P=0.04).

Conclusion: The finding of this study indicated that for the first time, there was a significant association between miR-143 polymorphisms and the risk of endometriosis. Further studies in other populations with larger samples are needed to confirm these findings.

Keywords: Infertility, Endometriosis, MiR-143, Polymorphism, miRNA
Full-Text [PDF 308 kb]   (742 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/11/13 | Accepted: 2016/11/13 | Published: 2016/11/13
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