:: Volume 20, Issue 5 (Bimonthly 2016) ::
Feyz 2016, 20(5): 435-440 Back to browse issues page
The prevalence of Set1 A and Set1 B genes in clinical Shigella sonnei strains using multiplex-PCR
Mina Sadeghifard , Kumarss Amini , Javad Nasr , Ramak Yahyaraeyat
Department of Animal Science, Saveh Branch, Islamic Azad University, Saveh I. R. Iran. , kamini@iau-saveh.ac.ir
Abstract:   (3899 Views)

Background: Shigella spp have been reported as the common cause of mortality in children. Recent studies in Iran have shown that Shigella sonnei is an important specie for nosocomial infection. The set1A and set1B are two critical virulence factors for human disease. The aim of this study was to evaluate the frequency of set1A and set1B genes in bacterial strains isolated from people with diarrhea dysentery using the multiplex-PCR (M-PCR) method.

Materials and Methods: A total 60 strains of Shigella sonnei were collected from Children Medical Center (Tehran, Iran). Then, these isolates were identified by standard biochemical and culture phenotypic methods. Detection of both of set1B and set1A virulence genes was carried out by M-PCR. Antibacterial susceptibility testing to Amoxicillin, Clavulanic acid, Tetracycline, Cefixime, Ceftriaxone, Cefepime and Cotrimoxazole was performed according to CLSI guideline using disk diffusion method.

Results: All 60 isolates were identified as S. sonnei. The highest resistance was observed to both of Cotrimoxazole and Cefixime antibiotics, while 95% of strains were sensitive to Tetracycline. The prevalence of Set1A and set1B virulence genes were 18 (30%) and 0 (0%) strains, respectively.

Conclusion: Nowadays, the increase of microbial resistance to antibiotics is a global problem which is caused by indiscriminate use of drugs. The results showed that the prevalence of the gene in the sample set could be a criterion for direct detection of Shigella in the sample.
Keywords: Shigella sonnei, Set1 A and Set1 B genes, Multiplex PCR
Full-Text [PDF 187 kb]   (1861 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/11/13 | Revised: 2017/07/29 | Accepted: 2016/11/13 | Published: 2016/11/13
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