:: Volume 20, Issue 5 (Bimonthly 2016) ::
Feyz 2016, 20(5): 435-440 Back to browse issues page
The prevalence of Set1 A and Set1 B genes in clinical Shigella sonnei strains using multiplex-PCR
Mina Sadeghifard, Kumarss Amini *, Javad Nasr, Ramak Yahyaraeyat
Department of Animal Science, Saveh Branch, Islamic Azad University, Saveh I. R. Iran. , kamini@iau-saveh.ac.ir
Abstract:   (2176 Views)

Background: Shigella spp have been reported as the common cause of mortality in children. Recent studies in Iran have shown that Shigella sonnei is an important specie for nosocomial infection. The set1A and set1B are two critical virulence factors for human disease. The aim of this study was to evaluate the frequency of set1A and set1B genes in bacterial strains isolated from people with diarrhea dysentery using the multiplex-PCR (M-PCR) method.

Materials and Methods: A total 60 strains of Shigella sonnei were collected from Children Medical Center (Tehran, Iran). Then, these isolates were identified by standard biochemical and culture phenotypic methods. Detection of both of set1B and set1A virulence genes was carried out by M-PCR. Antibacterial susceptibility testing to Amoxicillin, Clavulanic acid, Tetracycline, Cefixime, Ceftriaxone, Cefepime and Cotrimoxazole was performed according to CLSI guideline using disk diffusion method.

Results: All 60 isolates were identified as S. sonnei. The highest resistance was observed to both of Cotrimoxazole and Cefixime antibiotics, while 95% of strains were sensitive to Tetracycline. The prevalence of Set1A and set1B virulence genes were 18 (30%) and 0 (0%) strains, respectively.

Conclusion: Nowadays, the increase of microbial resistance to antibiotics is a global problem which is caused by indiscriminate use of drugs. The results showed that the prevalence of the gene in the sample set could be a criterion for direct detection of Shigella in the sample.

Keywords: Shigella sonnei, Set1 A and Set1 B genes, Multiplex PCR
Full-Text [PDF 187 kb]   (691 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/11/13 | Accepted: 2016/11/13 | Published: 2016/11/13
1. Wang XY, DU L, Seidlein LV, Xu ZY, Zhang YL, Hao ZY, et al. Occurrence of Shigellosi in the young and elderly in rural china: results of a 12-month population based surveillance study. Am J Trop Med Hyg 2005; 73(2): 416–22.
2. Swapan KN. Shigellosis. J Microbiol 2005; 43(2): 133-43.
3. Pitisuttithum P, Islam D, Chamnanchanunt S, Ruamsap N, Khantapura P, Kaewkungwal J. clinical trial of an oral live Shigella sonnei vaccine candidate, WRSS1, in Thai. Clin Vaccine Immunol 2016; 23(7): 564-75.
4. Odumosu O, Nicholas D, Yano H, Langridge W. AB toxins: a paradigm switch from deadly to desirable. Toxins (Basel) 2010; 2(7): 1612-45.
5. Sur D, Ramamurthy T, Deen J, Bhattacharya S. Shigellosis: Challenges & management issues. Indian J Med Res 2004; 120(5): 454.
6. Dennehy PH. Acute diarrheal disease in children: Epidemiology, prevention, and treatment. Infect Dis Clin North Am Title 2005; 19(3): 585-602.
7. Lamba K, Nelson JA, Kimura AC, Poe A, Collins J, Kao AS, et al. Shiga Toxin 1–Producing Shigella sonnei Infections, California, United States, 2014–2015. Emerg Infect Dis 2016; 22(4): 679-86.
8. Sansonetti PJ. Microbes and microbial toxins: paradigms for microbial-mucosal interactions III. Shigellosis: from symptoms to molecular pathogenesis. Am J Physiol Gastrointest Liver Physiol 2001; 280(3): G319-23.
9. Gray MD, Lacher DW, Leonard SR, Abbott J, Zhao S, Lampel KA, et al. Prevalence of Stx-producing Shigella species isolated from French travellers returning from the Caribbean: an emerging pathogen with international implications. Clin Microbiol Infect Epub 2015; 14.
10. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. M100-S25 2015; 35(3): 44-50.
11. Mandal J, Poonambath DK, Bhosale NK, Das A. Novel strain of Shigella dysenteriae serotype 7 from India. New Microbes New Infect 2015; 7: 97-9.
12. Butler T. Hemolytic uremic syndrome during shigellosis. Trans R Soc Trop Med Hyg 2012; 106(7): 395–9.
13. Ghandian S, Sattari M, Nikbin VS, Aslani MM. Study of antibiotic susceptibility pattern and presence of ipaH gene among Shigella strains isolated from selected provinces in Iran. Pathol Reas 2011; 14(1): 81-8.
14. Mandomando I, Jaintilal D, Pons MJ, Vallès X, Espasa M, Mensa L, et al. Antimicrobial susceptibility and mechanisms of resistance in Shigella and Salmonella isolates from children under five years of age with diarrhea in rural mozambique. Antimicrob Agents chemother 2009; 53(6): 2450-54.
15. Thong KL, Hoe SL, Puthucheary SD, Yasin RM. Detection of virulence genes in Malaysian Shigella species by Multiplex PCR assay. BMC Infect Dis. 2005; 5(1): 1.
16. Vargas M, Gascon J, De Anta MTJ, Vila J. Prevalence of shigella enterotoxins 1 and 2 among Shigella strains isolated from patients with traveler's diarrhea. J Clin Microbiol 1999; 37(11): 3608-11.

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