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:: Volume 20, Issue 5 (Bimonthly 2016) ::
Feyz 2016, 20(5): 413-419 Back to browse issues page
The effect of alcoholic extract of Stachys lavandulifolia Vahl leave on alpha motoneuron regeneration of anterior horn in a rat model of sciatic nerve compression
Maryam Tehranipour *, Fatemeh Attarian
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, I. R. Iran. , maryam_tehranipour@mshdiau.ac.ir
Abstract:   (1739 Views)

Background: Peripheral nerve injury during accidents causes the degeneration of the cell body in anterior horn of the spinal cord. Sometimes the use of plant extracts may be effective in regenerative process of these injuries. Stachys lavandulifolia of the Laminacea family has antioxidant and anti-apoptotic effects. The aim of this study was to investigate the regenerative effect of alcoholic extract of Stachys lavandulifolia leave on spinal cord alpha motoneuron in a rat model of sciatic nerve compression.

Materials and Methods: In this experimental study male Wistar rats (n=36) were divided randomly into six groups: Control, Compression, and Compression + Treatment (25, 50, 75, 100 mg/kg of extract). In order to induce compression, sciatic nerve of right leg was exposed to compression for 60 seconds using the locker pincers. Extract injection was done intraperitoneally in the first and second weeks after the compression. Then, 28 days after the compression and under profusion method, the samples were taken from lumbar spinal cord. The density of motoneurons was measured and compared using the dissector and stereological methods.

Results: According to the present results, the density of neurons in Compression group was decreased significantly compared to the Control (P<0.001) one. Moreover, Neuronal density    was increased significantly in all Treatment groups (25, 50, 75, and 100 mg/kg doses) compared to the Compression group (P<0.01).

Conclusion: The alcoholic extract of Stachys lavandulifolia leave has neuro-protective effect on anterior horn neurons of the spinal cord after injury. Probably, this effect is related to the existence of antioxidant and anti-inflammatory factors in alcoholic extract that induces the neuronal regeneration.

Keywords: Alpha motoneuron, Compression, Regeneration, Sciatic nerve, Stachys Lavandulifolia
Full-Text [PDF 330 kb]   (483 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/11/13 | Accepted: 2016/11/13 | Published: 2016/11/13
References
1. Ferrer I, Planas AM. Signaling of cell death and cell survival following focal cerebral ischemia: life and death struggle in the penumbra. J Neuropathol Experimental Neurol 2003; 62(4): 329-39.
2. Vargas ME, Barres BA. Why Is Wallerian Degeneration in the CNS So Slow? Ann Rev Neurosci 2007; 30: 153-79.
3. Li SX, Cui N, Zhang C, Zhao X, Yu SF, Xie KQ. Effect of subchronic exposure to acrylamide induced on the expression of bcl-2, bax and caspase-3 in the rat nervous system. Toxicology 2006; 217(1): 46-53.
4. McPhail LT, Stirling DP, Tetzlaff W, Kwiecien JM, Ramer MS. The contribution of activated phagocytes and myelin degeneration to axonal retraction/dieback following spinal cord injury. Eur J Neurosci 2004; 20(8): 1984-94.
5. Yiu G, He Z. Glial inhibition of CNS axon regeneration. Nat Rev Neurosci 2006; 7(8): 617-27.
6. Liu D, Bao F, Ling X. Reactive species and apoptotic cell death in spinal cord injury. Neurochemistry 2003; 87(144).
7. Hayashi T, Sakurai M, Abe K, Sadahiro M, Tabayashi K, Itoyama Y. Apoptosis of motor neurons with induction of caspases in the spinal cord after ischemia. Stroke1998; 29(5): 1007-13.
8. Tehranipour M, Javadmoosavi B. The neuroprotective effect of alcoholic extract of Cannabis sativa on neuronal density of spinal cord alpha motoneurons after sciatic nerve injury in rats. J Shahid Sadoughi Univ Med Sci 2011; 19(3): 339-49.
9. Jalali M, Tehranipour M, Mahdavi Shahri N. Effect of alcoholic extract of Nigella sativa seed on alpha motor neurons density of spinal cord following sciatic nerve compression in rats. J Gorgan Univ Medical Sci 2014; 15(4): 29-34. [in Persian]
10. Tehranipour M, Mollashahi M, Javadmoosavi B. Effect of ethanolic extract of pod Prosopis farcta plant on neuronal density of anterior horn following sciatic nerve compression in Rats. J Gorgan Univ Med Sci 2013; 14(4): 39-43.
11. Razavi M, Tehranipour M, Khayatzadeh J. Effects of aqueous extract of Salvia chloroleuca leaves on degeneration of alpha motoneurons in spinal cord after sciatic nerve compression in rat. J Shahrekord Univ Med Sci 2014; 16(2): 22-30. [in Persian]
12. Alikhanzadeh M, Tehranipour M, Khayatzadeh J. The study of effect of aquatic extracts of achillea biebersteinii leave on repair alpha motoneurons after sciatic nerve injury in rat. J Shahrekord Univ Med Sci 2013; 15(4): 16-25. [in Persian]
13. Babakhanlo P, Mirzai M, Sefidkon F, Ahmadi L, Barazaneh M, Asgari F. Medical and aromatic plant research in state of forests and rangelands. 1st ed. Ministry of Jahad-e-Agriculture; 1998. p. 64-82.
14. Maleki N, Garjani A, Nazemiyeh H, Nilfouroushan N, Eftekhar Sadat AT, Allameh Z, et al. Potent anti-inflammatory activities of hydroalcoholic extract from aerial parts of Stachys inflata on rats. J Ethnopharmacol 2001; 75(2–3): 213-8.
15. Rabbani M, Sajjadi SE, Jalali A. Hydroalcohol extract and fractions of Stachys lavandulifolia vahl: effects on spontaneous motor activity and elevated plus-maze behaviour. Phytotherapy Res 2005; 19(10): 854-8.
16. Sereshti M, Yousofi DH, Zebardast N, Rafean M, Manouchehri NK, Yousefi H. Effect of ethanolic and watery extract of aerial parts of stachys lavandulifolia on trichomonas vaginalis, in vitro. J Med Plants 2012; 11(8): 159-65. [in Persian]
17. Jafarzadeh L, Rafieian-Kopaei M, Ansari Samani R, Asgari A. The effect of hydroalcoholic extract of Stachys lavandulifolia vahl on pregnant mice. Excli J 2012; 11: 357-62.
18. Jafarzadeh L, Asgari A, Golshan-Iranpoor F, Kheiri S, Parvin N, Rafieian M, et al. Abortificient effects of Stachys lavandulifolia Vahl in mice. J Shahrekord Uuniv Med Sci 2010; 11(4): 26-31. [in Persian]
19. Cicchetti E, Chaintreau A. Comparison of extraction techniques and modeling of accelerated solvent extraction for the authentication of natural vanilla flavors. J Separation Sci 2009; 32(11): 1957-64.
20. Behnam-Rasouli M, Nikravesh MR, Mahdavi Shahri N, Tehranipour M. Post-operative time effects after sciatic nerve crush on the number of alpha motoneurons, using a stereological counting method (Disector). Iran Biomedical J 2000; 4(1): 45-9.
21. Tehranipour M, Ghadamyari T. The effects of root aquatic extract of Salvia staminea on neuronal density of alpha motoneurons in spinal cord anterior horn after sciatic nerve compression in rats. J Biol Sci 2010; 10(1): 48-52.
22. Khayyatzade J. The protective effects of curcuma longa total extract on spinal cord neuroglia cell degeneration after the sciatic nerve compression in rats. Arak Med Univ J 2010; 13(1): 83-9. [in Persian]
23. Tehranipour M, Kabiri M. The effect of exogenous testosterone administration on peripheral nerves regeneration after sciatic nerve compression in rat. J Biol Sci 2009; 9(7): 692-706.
24. Sterio DC. The unbiased estimation of number and sizes of arbitrary particles using the disector. J Microsc1984; 134(2): 127-36.
25. Guertin AD, Zhang DP, Mak KS, Alberta JA, Kim HA. Microanatomy of Axon/Glial Signaling during Wallerian Degeneration. J Neurosci 2005; 25(13): 3478-87.
26. Kinugasa T, Ozaki S, Hamanaka S, Kudo N. The effects of sciatic nerve axotomy on spinal motoneurons in neonatal Bax-deficient mice. Neurosci Res 2002; 44(4): 439-46.
27. Van der Stelt M, Di Marzo V. Cannabinoid receptors and their role in neuroprotection. Neuromol Med 2005; 7(1-2): 37-50.
28. Sagredo O, García-Arencibia M, de Lago E, Finetti S, Decio A, Fernández-Ruiz J. Cannabinoids and Neuroprotection in Basal Ganglia Disorders. Mol Neurobiol 2007; 36(1): 82-91.
29. Sarris J, McIntyre E, Camfield DA. Plant-Based Medicines for Anxiety Disorders, Part 2: A Review of Clinical Studies with Supporting Preclinical Evidence. CNS Drugs 2013; 27(4): 301-19.
30. Rahzani K, Malekirad A, Zeraatpishe A, Hosseini N, Seify S, Abdollahi M. Anti-oxidative stress activity of stachys lavandulifolia aqueous extract in human. Cell J 2013; 14(4): 314-7.
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Tehranipour M, Attarian F. The effect of alcoholic extract of Stachys lavandulifolia Vahl leave on alpha motoneuron regeneration of anterior horn in a rat model of sciatic nerve compression . Feyz. 2016; 20 (5) :413-419
URL: http://feyz.kaums.ac.ir/article-1-3172-en.html


Volume 20, Issue 5 (Bimonthly 2016) Back to browse issues page
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