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:: Volume 21, Issue 6 (Bimonthly 2017) ::
Feyz 2017, 21(6): 562-568 Back to browse issues page
Prevalence of cagA, cagT, cagE, vacA and hrgA genes in Helicobacter pylori strains isolated from patients with gastric cancer in Karaj city, 2016
Elham Ahmadi , Kumarss Amini *, Mojtaba Sadeh
Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, I. R. Iran. , dr_kumarss_amini@yahoo.com
Abstract:   (330 Views)
Background: It is estimated that approximately half of the planet's population is infected with Helicobacter pylori and 70%-60% of the infections in the Western countries are caused by cagA-positive strains. The aim of this study was to determine the frequency of cagA, cagT, cagE, vacA and hrgA genes in H. pylori isolated from patients with gastric cancer.
Materials and Methods: A total of 50 non-repetitive biopsy samples were collected from patients undergoing endoscopy in the endoscopic center of the Shahid Fayaz Hospital in Karaj. The presence of cagA, cagT, cagE, vacA and hrgA genes was determined using the multiplex PCR method.
Results: Of the 50 gastric biopsies, 44 samples (88%) were positive for the presence of various virulence genes. The molecular analysis of virulence factors showed that the prevalence rates of cagA, cagT, cagE, vacA and hrgA genes were 16 (32%), 8 (16%), 13 (26%), 7 (14%) and 17 (34%), respectively. There was a significant relationship between sex, smoking and gastric ulcer with some genes, but no significant relationship was found between the family history and age group with any of the genes.
Conclusion: The presence of various pathogenic genes has a significant effect on gastric ulcer, duodenal ulcer and gastric cancer. The effects of other genes, such as hrgA, are important in tissue damage and inflammatory responses.
Keywords: Helicobacter pylori, cagA, cagT, cagE, vacA, hrgA
Full-Text [PDF 312 kb]   (49 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/11/13 | Accepted: 2017/09/25 | Published: 2017/12/12
1. Huang JY, Sweeney EG, Sigal M, Zhang HC, Remington SJ, Cantrell MA, et al. Chemodetection and destruction of host urea allows Helicobacter pylori to locate the epithelium. Cell Host Microbe 2015; 18(2):147–56.
2. Sigal M, Rothenberg ME, Logan CY, Lee JY, Honaker RW, Cooper RL, Passarelli B et al. Helicobacter pylori activates and expands Lgr5+ stem cells through direct colonization of the gastric glands. Gastroenterology 2015; 148 (7):1392–404.
3. Müller SA, Pernitzsch SR, Haange SB, Uetz P, von Bergen M, Sharma CM, et al. Stable isotope labeling by amino acids in cell culture based proteomics reveals differences in protein abundances between spiral and coccoid forms of the gastric pathogen Helicobacter pylori. J Proteomics 2015; 126:34–45.
4. De Bruyne E, Ducatelle R, Foss D, Sanchez M, Joosten M, Zhang G, et al. Oral glutathione supplementation drastically reduces Helicobacter-induced gastric pathologies. Sci Rep 2016; 6:20169.
5. [5] Hammond CE, Beeson C, Suarez G, Peek RM, Backert S, Smolka AJ. Helicobacter pylori virulence factors affecting gastric proton pump expression and acid secretion. Am J Physiol Gastrointest Liver Physiol 2015; 309(3): G193–201.
6. Zhang L, Wu WK, Gallo RL, Fang EF, Hu W, Ling TK, et al. Critical role of antimicrobial peptide cathelicidin for controlling Helicobacter pylori survival and infection. J Immunol 2016; 196(4): 1799–809.
7. Jung SW, Lee SW. The antibacterial effect of fatty acids on Helicobacter pylori infection. Korean J Intern Med 2016; 31(1): 30-5
8. Gaddy JA, Radin JN, Cullen TW, Chazin WJ, Skaar EP, Trent MS, et al. Helicobacter pylori resists the antimicrobial activity of calprotectin via lipid A modification on and associated biofilm formation. MBio 2015; 6(6):e01349-15.
9. Pachathundikandi SK, Lind J, Tegtmeyer N, El-Omar EM, Backert S. Interplay of the gastric pathogen Helicobacter pylori with toll-like receptors. Biomed Res Int 2015; 2015: 192420.
10. Nagashima H, Iwatani S, Cruz M, Jiménez Abreu JA, Uchida T, Mahachai V, et al. Toll-like receptor 10 in Helicobacter pylori infection. J Infect Dis 2015; 212(10):1666–76.
11. Pachathundikandi SK, Backert S. Differential expression of IL-1β during Helicobacter pylori infection of TLR2-and TLR10-expressing HEK293 cell lines. J Infect Dis 2016; 214(1): 166–7.
12. Obayashi N, Ohtsuka Y, Hosoi K, Ikuse T, Jimbo K, Aoyagi Y, et al. Comparison of gene expression between pediatric and adult gastric mucosa with Helicobacter pylori infection. Helicobacter 2016; 21(2): 114–23.
13. Kienesberger S, Cox LM, Livanos A, Zhang XS, Chung J, Perez-Perez GI, et al. Gastric Helicobacter pylori infection affects local and distant microbial populations and host responses. Cell Rep 2016; 14(6):1395–1407.
14. Essawi T, Hammoudeh W, Sabri I, Sweidan W, Farraj MA. Determination of Helicobacter pylori Virulence Genes in Gastric Biopsies by PCR. ISRN Gastroenterology 2013; 4: 1-4.
15. Chomvarin C, Namwat W, Chaicumpar K, Mairiang P, Sangchan A, Sripa B, et al. Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA and babA2 genotypes in Thai dyspeptic patients. Int J Infect Dis 2008; 12(1): 30-6.
16. Aghajani M, Abbasian M. Association of helicobacter pylori infection and its cagA-positive strains with Coronary heart disease (CHD). Iran South Med J 2002; 5(1): 56-62.
17. Molaei M, Foroughi F, Mashayekhi R, Haghazali M, Zojaji H, Jafari F, et al. CagA status and VacA subtypes of Helicobacter pylori in relation to histopathologic findings in Iranian population. Indian J Pathol Microbiol 2010; 53(1): 24-7.
18. Dousti A, Rahimian Gh, Nasiri J, Yavari Foroshani P. Identification the frequency of cytotoxin associated gene in H. pylori strain isolated form biopsy samples in Shahrekord. J Armaghan Danesh 2007; 29(1):12-21.
19. Douraghi M, Mohamadi M, Shirazi M.H, Esmaili M. Evaluation of cytotoxin associated gene with gastric disorders in H. pylori infected patients. Iran J Med Microbiol 2008; 2(1): 31-36. [In Persian]
20. Abdollahi H, Shokoohi M, Savari M. The prevalence of Helicobacter pylori babA2, iceA1 and iceA2 genes and their association with clinical outcomes in patients with chronic gastritis, ulcerative diseases and non-ulcer dyspepsia in south east of Iran. Jundishapur J Microbiol 2013; 6(4): e4739.
21. Talebi Bezmin Abadi A, Taghvaei T, Mohabbati Mobarez A, Vaira G, Vaira D. High correlation of babA 2-positive strains of Helicobacter pylori with the presence of gastric cancer. Intern Emerg Med 2013; 8(6): 497–501.
22. Vannarath S, Vilaichone RK, Rasachak B, Mairiang P, Yamaoka Y, Shiota S, et al. Virulence genes of Helicobacter pylori in gastritis, peptic ulcer and gastric cancer in Laos. Asian Pac J Cancer Prev 2014; 15(20): 9027-31.
23. Koehler CI, Mues MB, Dienes HP, Kriegsmann J, Schirmacher P, Odenthal M. Helicobacter pylori genotyping in gastric adenocarcinoma and MALT lymphoma by multiplex PCR analyses of paraffin wax embedded tissues. Mol Pathol 2003; 56(1): 36–42.
24. Ye H, Liu H, Attygalle A, Wotherspoon AC, Nicholson AG, Charlotte F, et al. Variable frequencies of t(11;18)(q21;q21) in MALT lymphomas of different sites: significant association with CagA strains of H. pylori in gastric MALT lymphoma. Blood 2003; 102(3):1012-8.
25. Hosseini E, Poursina F, Van de Wiele TV, Ghasemian Safaei HG, Adibi P. Helicobacter pylori in Iran: A systematic review on the association of genotypes and gastroduodenal diseases. J Res Med Sci 2012; 17(3): 280–92.
26. Fakher Yasseri H. Determination of Helicobacter pylori Prevalence in Histologic Gastritis And Intestinal Metaplasia and Related to Age and Sex Study on 576 Patients with Nonulcer Dyspepsia at Endascopy Department of Firozgar Hospital. J Iran Univ Med Sci 2002; 9(30): 379-88. [in Persian]
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Ahmadi E, Amini K, Sadeh M. Prevalence of cagA, cagT, cagE, vacA and hrgA genes in Helicobacter pylori strains isolated from patients with gastric cancer in Karaj city, 2016 . Feyz. 2017; 21 (6) :562-568
URL: http://feyz.kaums.ac.ir/article-1-3171-en.html

Volume 21, Issue 6 (Bimonthly 2017) Back to browse issues page
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