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:: Volume 20, Issue 4 (Bimonthly 2016) ::
Feyz 2016, 20(4): 376-382 Back to browse issues page
Molecular identification of virulence genes in clinical Salmonella typhimurium strains using the multiplex- PCR method and their antibiotic resistance profile
Mohammad Arjmand-Asl , Kumarss Amini *
Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, I. R. Iran. , dr_kumarss_amini@yahoo.com
Abstract:   (1598 Views)

Background: Salmonella as an enteric, gram negative, facultative anaerobic, obligate parasite causes food poisoning in human. The aim of present study was to identify the virulence genes stn, sopB, slyA, spvc and Phop/Q in Salmonella typhimurium strains isolated from clinical specimens using multiplex PCR method and also the determination of their antibiotic resistance profile.

Material and methods: In this cross-sectional study during a 12 month period (April to March 2015) a total of 60 Salmonella typhimurium isolates were collected from Shariati hospital (Tehran, Iran). After the identification of strains, the antimicrobial susceptibility test   was done using the disk diffusion agar test on Müller-Hinton agar media according to the Clinical and laboratory standards institute (CLSI) guidelines. Then the presence of virulence genes (e.g.  stn, sopB, slyA, spvc and Phop/Q ) were identified using multiplex PCR method.

Results: These findings obtained from isolates showed the highest sensitivity to the  Imipenem, Gentamicin, and Trimethoprim antibiotics . Distribution analysis of virulence for genes slyA, stn, sopb, Phop/Q showed  the frequency of  23.3%, 30%, 3.33%5 and 43.3%, respectively. The spvC gene is not seen in any isolates.

Conclusion: The results of this study indicate that the prevalence of virulence genes in clinical Salmonella Typhimurium isolates can serve as an alarm for the prevalence of these genes to the other Salmonella serotypes. As the Salmonella virulence genes are located on the Salmonella pathogenicity islands, so the high prevalence of these genes can help to the dissemination of islands among the Salmonella strains.

Keywords: Salmonella Typhimurium, Virulence genes, Multiplex-PCR
Full-Text [PDF 367 kb]   (1167 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/10/15 | Accepted: 2016/10/15 | Published: 2016/10/15
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Arjmand-Asl M, Amini K. Molecular identification of virulence genes in clinical Salmonella typhimurium strains using the multiplex- PCR method and their antibiotic resistance profile. Feyz. 2016; 20 (4) :376-382
URL: http://feyz.kaums.ac.ir/article-1-3139-en.html

Volume 20, Issue 4 (Bimonthly 2016) Back to browse issues page
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