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:: Volume 20, Issue 4 (Bimonthly 2016) ::
Feyz 2016, 20(4): 331-339 Back to browse issues page
Comparing the antifungal effects of gold and silver nanoparticles isolated from patients with vulvovaginal candidiasis in-vitro
Ladan Rahimzadeh-Torabi *, Monir Doudi, Noshin Naghsh, Zeynab Golshani
Department of Microbiology, Faculty of Basic Science, Falavarjan Branch, Islamic Azad University, Isfahan, I. R. Iran. , negar.rahimz@gmail.com
Abstract:   (1871 Views)

Background: In recent years the emergence of resistance to opportunistic fungi (e.g. Candida albicans) has dramatically increased worldwide. The toxicity of antibacterial drugs and the occurrence of resistance to fungi and the resultant drug interactions make it necessary to assess new drugs. The aim of this study was to compare the antifungal effects of gold and silver nanoparticles on patients with vulvovaginal candidiasis.

Materials and Methods: In order to identify the samples, the conventional mycological methods (morphology on corn meal/chrome agar and germ tube production) were utilized. To evaluate the antifungal effects of spherical silver and gold nanoparticles the diameter of inhibitory zone was measured using disk diffusion method. In addition, the minimum fungicidal concentration (MIC) and Minimum inhibitory concentration (MFC) of samples were determined by microdilution method.

Results: Among the total identified isolates of Candida albicans (C. albicans), 58 samples were inhibited using spherical silver and gold nanoparticles with 10 nm diameter. The inhibitory zone diameter was between 0-19 mm. The MIC and MFC for silver and gold nanoparticles were 21.31±11.40; 32.15±25.77 ppm and 16.68±9.37; 7.93±4.72 ppm, respectively.

Conclusion: The results of the present investigation showed that spherical gold and silver nanoparticles with 10 nm diameter have some antifungal effects against the Candida albicans. Probably in future the silver and gold nanoparticles have the potential of treating the vulvovaginal candidiasis.

Keywords: Gold Nanoparticles, Silver nanoparticles, Vulvovaginal candidiasis, Candida albicans
Full-Text [PDF 354 kb]   (1309 Downloads)    
Type of Study: Research | Subject: General
Received: 2016/10/10 | Accepted: 2016/10/10 | Published: 2016/10/10
1. Vrablik J, Masata J, Jedlickova A, Hajicková M. [Prospective study the prevalence of different candida strains and their sensitivity to different antimycotic treatment in women with vulvovaginal candidiasis]. Ceska Gynekol 2007; 72(1): 27-32.
2. Kent HL. Epidemiology of vaginitis. Am J Obstet Gynecol 1991; 165(4 Pt 2): 1168-76.
3. Aalei B, Touhidi A. Prevalence of candida vaginits among symptomatic patients in kerman. J Qazvin Univ Med Sci 2000; 4(1): 42-8. [in Persian]
4. Mosadegh M, Dehmoubed Sharifabadi A, Nasiri P, Esmaeili S, Naghibi F. The study of phytochemical, antifungal and antibacterial effects of teucrium polium and cichourium intybus. Scientific J Kurdistan Univ Med Sci 2002; 7(1): 1-6. [in Persian]
5. Biggs WS, Williams RM. Common gynecologic infections. Prim Care 2009; 36(1): 33-51, viii.
6. Vacheva-Dobrevski R, Kovachev S, Nacheva A, Stoev S, Vasilev N. Comparative study of itraconazole and fluconazole therapy in vaginal candidosis. Akush Ginekol (Sofiia) 2004; 43(1): 20-3.
7. Quan M. Vaginitis: meeting the clinical a challenge. Clin Cornerstone 2000; 3(1): 36-47.
8. Handa V, Stice CW. Fungal culture findings in cyclic vulvitis. Obstet Gynecol 2000; 96(2): 301-3.
9. Oriel JD, Partridge BM, Denny MJ, Coleman JC. Genital yeast infections. Br Med J 1972; 4(5843): 761-4.
10. Cristiano L, Rampello S, Noris C, Valota V. Bacterial vaginosis: prevalence in an Italian population of asymptomatic pregnant women and diagnostic aspects. Eur J Epidemiol 1996; 12(4): 383-90.
11. Ventolini G, Baggish MS, Walsh PM. Vulvovaginal candidiasis from non-albicans species: retrospective study of recurrence rate after fluconazole therapy. J Reprod Med 2006; 51(6): 475-8.
12. Khoursandi M, Modares Gilani M, Khosravi AR. Treatment and recurrence of vaginal candidiasis after oral & Vulvovaginal Candidiasis and intravaginal treatment. J Qazvin Univ medical Sci 2000; 4(2): 25-9. [in Persian]
13. Horowitz BJ. Mycotic vulvovaginitis: abroad overview. Am J Obstet Gynecol 1991; 165(4 Pt 2): 1188-92.
14. Faye-Kette YH, Kouassi AA, Sylla-Koko DF, Kacou-N'-Douba A, Akoua-Koffi G, Acho YB, et al. [Prevalence of 4 agents of sexually transmitted diseases in leukorrhea in Abidjan (Ivory Coast)]. Bull Soc Pathol Exot 1993; 86(4): 245-7.
15. Ferris DG, Hendrich J, Payne PM, Getts A, Rassekh R, Mathis D, et al. Office laboratorydiagnosis of vaginitis. Clinician-performed testscompared with a rapid nucleic acidhybridization test. J Fam Pract 1995; 41(6):575-81.
16. Richter SS, Galask RP, Messer SA, Hollis RJ, Diekema DJ, Pfaller MA. Antifungal susceptibilities of Candida species causing vulvovaginitis and epidemiology of recurrent cases. J Clin Microbiol 2005; 43(5): 2155-62.
17. Connor EE, Mwamuka J, Gole A, Murphy CJ, Wyatt MD. Gold nanoparticles are taken up by human cells but donot cause acute cytotoxicity. Small 2005; 1(3): 325–7.
18. Salata O. V. (2004).Application & Properties of nanoparticles in biology and medicine.j. nano biotechnology.2 (3) : 1-8.
19. Pfaller M, Messer S. Coparision of visual and spectrophotometris methods of MIC and point determinations by using broth microdilution methods to test five antifungal agents including the new triazol. J Clin Microbiol 1995; 33: 1094-7.
20. Nccls. Methods for antifungal disc diffusion suseptibility testing of yeast, Approved Guideline NCELS Document M44-A. NCCLS. Wayne. Pennsylranias; 2004.
21. Chwalibog A, Sawosz E, Hotowy A, Szeliga J, Mitura S, Mitura K, et al. Visualization of interaction between inorganic nanoparticles and bacteria or fungi. Int J Nanomedicine 2010; 6(5): 1085-94.
22. Zhao G, Stevens SE Jr. Multiple parameters for the comprehensive evaluation of the susceptibility of Escherichia coli to the silver ion. Biometals 1998; 11(1): 27–32.
23. Jebali A, Hajjar FH, Pourdanesh F, Hekmati‌moghadam S, Kazemi B, Masudi M, et al. Silver and gold nanostructures: antifungal property of different shapes of these nanostructures on Candida species. Med Mycol 2014; 52(1): 65-72.
24. Martinez-Gguterrez F, Olive PL, Banuelos A. Characterization and evalution of antimicrobial and cytotoxic effect of silver and titanium nanoparticles. Nanomedicine 2010; 6(5): 681-8.
25. Zhang L, Pornpattananangkul D, Hu CM, Huang CM. Developmant of nanoparticles for antimicrobial drug delivery. Curr Med Chem 2010; 17(6): 585-94.
26. Zheng Y, Sache L. Gold nanoparticles enhance DNA damage induced by anti-cancer drugs and radiation. Radiat Res 2009; 172(1): 114–9.
27. Kim KJ, Sung WS, Suh BK, Moon SK, Choi JS, Kim JG, et al. Antifungal activity and mode of action of silver nano-particles on Candida albicans. Biometals 2009; 22(2): 235-42.
28. Wani IA, Ahmad T, Manzoor N. Size and shape dependant antifungal activity of gold nanoparticles: a case study of Candida. Colloids Surf B Biointerfaces 2013; 101(1): 162-70.
29. Zawrah MF, El-Moez SA, Center D. Antimicrobial activities of gold nanoparticles against major foodborne pathogens. Life Sci J 2011; 8(4): 37-44.
30. Nasrollahi A, Pourshamsian KH, Mansourkiaee P. Antifungal activity of silver nanoparticles on some os fungi. Int J Nano Dim 2011; 1(3): 233-9.
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Rahimzadeh-Torabi L, Doudi M, Naghsh N, Golshani Z. Comparing the antifungal effects of gold and silver nanoparticles isolated from patients with vulvovaginal candidiasis in-vitro. Feyz. 2016; 20 (4) :331-339
URL: http://feyz.kaums.ac.ir/article-1-3130-en.html

Volume 20, Issue 4 (Bimonthly 2016) Back to browse issues page
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