:: Volume 20, Issue 4 (Bimonthly 2016) ::
Feyz 2016, 20(4): 306-314 Back to browse issues page
The protective effect of fenugreek (Trigonella foenum-graecum) seed powder on acetaminophen-induced hepatotoxicity in male rats
Aghileh Mohammadzadeh , Ali Gol , Abdoreza Javadi
Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, I. R. Iran. , agol@mail.uk.ac.ir
Abstract:   (3926 Views)

Background: Acetaminophen overdose is the most prevalent cause of drug-induced liver injury. Many traditional treatments are recommended as the complementary and alternative options for treating various diseases. In this study the effect of fenugreek seed powder on the acetaminophen-induced hepatotoxicity is investigated.

Materials and Methods: In this experimental study, male Wistar rats (no=24, weight 250-280 g) were divided into four groups: Control (C), Fenugreek seed powder (T); Acetaminophen (A); and Acetaminophen+Fenugreek seed powder (A+T). After 24 h of fasting, A and A+T groups received Acetaminophen 1000 mg/kg by gavage, and the other two groups received  Saline. After six hours, C and A groups received Saline, whereas T and A+T groups received Fenugreek seed powder 1000 mg/kg via gavage. Twelve hours after the second gavage, the rats were sacrificed and the liver tissue was fixed with formalin and assessed. Results were analyzed using ANOVA and Tukey post-test.

Results: Serum ALT and AST levels were significantly increased in group A compared to C (P<0.001 and 0.01, respectively). In addition, Acetaminophen resulted in liver tissue injury (necrosis, apoptosis, bleeding, and hyperemia). Treatment with fenugreek reversed the changes toward normal.

Conclusion: Fenugreek can have protective effects on hepatic injury induced by Acetaminophen.
Keywords: Fenugreek Powder, Acetaminophen, Hepatotoxicity, Rat
Full-Text [PDF 886 kb]   (1446 Downloads)    
Type of Study: Research | Subject: General
Received: 2016/10/10 | Revised: 2017/08/1 | Accepted: 2016/10/10 | Published: 2016/10/10
References
1. Sun H, Chen L, Zhou W, Hu L, Li L, Tu Q, et al. The protective role of hydrogen-rich saline in experimental liver injury in mice. J Hepatol 2011; 54(3): 471- 80.
2. Larrey D. Epidemiology and individual susceptibility to adverse drug reactions affecting the liver. Sem Liv Dis 2002; 22(2): 145-55.
3. Davern TJ. Drug-induced liver disease. Clin Liver Dis 2012; 16(2): 231-45.
4. Kleiner DE. The pathology of drug-induced liver injury. Semin Liver Dis 2009; 29(4): 364-72.
5. Holt M, Ju C. Drug-induced liver injury. Handb Exp Pharmacol 2010; (196): 3- 27.
6. Aghababian RV. Essentials of emergency medicine. Burlington 2nd ed. Jones & Bartlett Publishers; 2011. p. 814.
7. Nelson SD, Tirmenstein MA, Rashed MS, Myers TG. Acetaminophen and protein thiol modification. Adv Exp Med Biol 1991; 283: 579- 88.
8. Nanji AA, Jokelainen K, Fotouhinia M, Rahemtulla A, Thomas P, Tipoe GL, et al. Increased severity of alcoholic liver injury in female rats: role of oxidative stress, endotoxin, and chemokines. Am J Physiol Gastrointest Liver Physiol 2001; 281(6): G1348-56.
9. Han D, Hanawa N, Saberi B, Kaplowitz N. Mechanisms of liver injury.III. Role of glutathione redox status in liver injury. Am J Physiol Gastrointest Liver Physiol 2006; 291(1): 1-7.
10. Jollow DJ, Mitchell JR, Potter WZ, Davis DC, Gillette JR, Brodie BB. Acetaminophen-induced hepatic necrosis. II. Role of covalent binding in vivo. J Pharmacol Exp Ther 1973; 187(1): 195–202.
11. Hamza RZ, Al-Harbi MS. Amelioration of paracetamol hepatotoxicity and oxidative stress on mice liver with silymarin and Nigella sativa extract supplements. Asian Pac J Trop Biomed 2015; 5(7): 521–31.
12. Ajith TA, Hema U, Aswathy MS. Zingiber officinale Roscoe prevents acetaminophen-induced acute hepatotoxicity by enhancing hepatic antioxidant status. Food Chem Toxicol 2007; 45 (11): 2267-72.
13. Yakubu N, Oboh G, Olalekan AA. Antioxidant and Hepatoprotective Properties of Tofu (Curdle Soymilk) against Acetaminophen-Induced Liver Damage in Rats. Biotechnol Res Int 2013; 2013: 230142.
14. Basu TK, Srichamroen A. Health Benefits of Fenugreek. In: Watson RR, Preedy VR editors. Bioactive Foods in Promoting Health. 1st ed. Academic Press; 2010. p. 425-35.
15. Kaviarasan S, Naik GH, Gangabhagirathi R, Anuradha CV, Priyadarsini KI. In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum graecum) seeds. Food Chem 2007; 103(1): 31-7.
16. Abou El-Soud NH, Khalil MY, Hussein JS, Oraby FSH, Farrag ARH. Antidiabetic effect of fenugreek alkaloid extract in streptozotocin-induced hyperglycemic rats. J Appl Sci Res 2007; 3(10): 1073–83.
17. Belguith-Hadriche O, Bouaziz M, Jamoussi K, El Feki A, Sayadi S, Makni-Ayedi F. Lipid lowering and antioxidant effects of an ethyl acetate extract of fenugreek seeds in high-cholesterol-fed rats. J Agric Food Chem. 2010; 58(4): 2116-22.
18. Mohan M, Kamble S, Gadhi P, Kasture S. Protective effect of Solanum torvum on doxorubicininduced nephrotoxicity in rats. Food Chem Toxicol 2010; 48(1): 436–40.
19. Adam GO, Rahman MM, Lee SJ, Kim GB, Kang HS, Kim JS, et al. Hepatoprotective effects of Nigella sativa seed extract against acetaminophen-induced oxidative Stress. Asian Pac J Trop Med 2016; 9(3): 221-7.
20. Song ZY, McClain CJ, Chen T. S-adenosylmethionine protects against acetaminophen-induced hepatotoxicity in mice. Pharmacology 2004; 71(4): 199-208.
21. Hsu CC, Lin CC, Liao TS, Yin MC. Protective effect of s-allyl cysteine and spropyl cysteine on acetaminophen-induced hepatotoxicity in mice. Food Chem Toxicol 2006; 44(3): 393-7.
22. Pari L, Suresh A. Effect of grape (Vitis vinifera L.) leaf extract on alcohol induced oxidative stress in rats. Food Chem Toxicol 2008; 46(5): 1627-34.
23. Saxena M, Shakya AK, Sharma N, Shrivastava S, Shukla S. Therapeutic Efficacy of Rosa damascena Mill. on Acetaminophen-Induced Oxidative Stress in Albino Rats. J Environ Pathol Toxicol Oncol 2012; 31(3): 193-201.
24. He M, Zhang S, Jiao Y, Lin X, Huang J, Chen C, et al. Effect and mechanisms of rifampin on hepatotoxicity of acetaminophen in mice. Food Chem Toxicol 2012; 50(9): 3142-9.
25. Abdel-Daim MM, Abd Eldaim MA, Hassan A G.A. Trigonella foenum-graecum ameliorates acrylamide-induced toxicity in rats: Roles of oxidative stress, proinflammatory cytokines, and DNA damage. Biochem Cell Biol 2015; 93(3): 192-8.
26. Sushma N, Devasena T. Aqueous extract of Trigonella foenum graecum (Fenugreek) prevents cypermethrin-induced hepatotoxicity and nephrotoxicity. Hum Exp Toxicol 2010; 29(4): 311-9.
27. Al-Wabel NA, Mousa HM, Omer OH, Abdel-Salam AM. Biological evaluation of aqueous herbal extracts and stirred yoghurt filterate mixture against alloxan-induced oxidative stress and diabetes in rats. Int J Pharmacol 2008; 1: 1–5.
28. Hassan AM, Khalil WKB, Ahmed KA. Effect of fenugreek oil extract on the efficiency of ovarian and liver tissues. Afr J Biotechnol 2006; 5: 477–83.
29. Khalil EAM. Biochemical and histopathological studies on the influence of aqueous extract of fenugreek seed (Trigonella foenum graecum) on alloxan diabetic male rats. Egypt J Hospital Med 2004; 15: 83–94.
30. Mitchell JR, Jollow DJ, Potter WZ, Davis DC, Gillette JR, Brodie BB. Acetaminophen- induced hepatic necrosis. I. Role of drug metabolism. J Pharmacol Exp Ther 1973; 187(1): 185-94.
31. Rietjens IM, Boersma MG, Haan Ld, Spenkelink B, Awad HM, Cnubben NH, et al. The pro-oxidant chemistry of thenatural antioxidants vitamin C, vitamin E, carotenoids and flavonoids. Environ Toxicol Pharmacol 2002; 11(3-4): 321- 33.
32. Yousef MI, Omar SA, El-Guendi MI, Abdelmegid LA. Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress impaired liver and kidney functions and haematotoxicity in rat. Food Chem Toxicol 2010; 48(11): 3246-61.
33. Moskaug JØ, Carlsen H, Myhrstad MC, Blomhoff R. Polyphenols and glutathione synthesis regulation. Am J Clin Nutr 2005; 81(1 Suppl): 277-83.
34. Myhrstad MC, Carlsen H, Nordström O, Blomhoff R, Moskaug JØ. Flavonoids increase the intracellular glutathione level by transactivation of the gamma glutamyl cysteine synthe-tase catalytical subunit promoter. Free Radic Biol Med 2002; 32(5): 386- 93.
35. Raucy JL. Regulation of CYP3A4 expression in human hepatocytes by pharmaceuticals and natural products. Drug Metab Dispos 2003; 31(5): 533- 9.
36. Lv Y, Zhang B, Xing G, Wang F, Hu Z. Protective effect of naringenin against acetaminopheninduced acute liver injury in metallothionein (MT)-null mice. Food Funct 2013; 4(2): 297-302.
37. Blazka ME, Elwell MR, Holladay SD, Wilson RE, Luster MI. Histopathology of acetaminophen-induced liver changes: role of interleukin 1 alpha and tumor necrosis factor alpha. Toxicol Pathol 1996; 24(2): 181-9.
38. Sakr SA, Abo-El-Yazid SM. Effect of fenugreek seed extract on adriamycin- induced hepatotoxicity and oxidative stress in albino rats. Toxicol Ind Health 2012; 28(10): 876-85.
39. Yousef MI, Omar SA, El-Guendi MI, Abdelmegid LA. Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat. Food Chem Toxicol 2010; 48(11): 3246- 61.

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