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:: Volume 19, Issue 6 (Bimonthly 2016) ::
Feyz 2016, 19(6): 457-467 Back to browse issues page
Apoptotic effects of silver nanoparticles coated with Zataria multiflora leaves extract on HepG2 cell line
Negar Khorasani, Javad Baharara *, Alireza Iranbakhsh, Tayebeh Ramezani
Mashhad Branch, Islamic Azad University , baharara78@gmail.com
Abstract:   (2373 Views)

Background: Silver nanoparticles (AgNPs) have antibacterial, anti-angiogenesis and anti-cancer activity. The purpose of this study was to examine the apoptotic effects of AgNPs coated with Zataria multiflora leaves extract on HepG2 liver cancer cells.

Materials and Methods: HepG2 cancer cell lines were treated with different concentrations of AgNPs for 24 and 48 hours. The viability of the cells and inhibitory concentration (IC50) were determined by the methylthiazol tetrazolium (MTT) assay. To evaluate the ability of AgNPs to induce apoptosis in HepG2 cells, the 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI), acridine orange-propidium iodide staining and flow cytometry analysis for annexin V- propidium iodide were used.

Results: The results of the MTT assay showed that AgNPs decreased the proliferation of HepG2 cells in dose- and time-dependent manner. The IC50 during the incubation times of 24 and 48 hours were determined 40 and 30 &mu;g/mL, respectively (P<0.05). The results of DAPI staining showed that AgNPs can lead to break down of nuclear DNA. Moreover, the acridine orange-propidium iodide staining and annexin V-propidium iodide showed that the percentage of apoptotic cells was increased in the treated cells.

Conclusion: Silver nanoparticles covered with Z. multiflora leaf extract have the ability to induce apoptosis in cancer cells HepG2 and they can be considered as a promising strategy for the treatment of liver cancer.

Keywords: Silver nanoparticles, Cancer, Apoptosis, Zataria multiflora
Full-Text [PDF 578 kb]   (1753 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/02/3 | Accepted: 2016/02/3 | Published: 2016/02/3
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Khorasani N, Baharara J, Iranbakhsh A, Ramezani T. Apoptotic effects of silver nanoparticles coated with Zataria multiflora leaves extract on HepG2 cell line. Feyz. 2016; 19 (6) :457-467
URL: http://feyz.kaums.ac.ir/article-1-2910-en.html


Volume 19, Issue 6 (Bimonthly 2016) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
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