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The effect of melittin on the inhibition of Rac1 expression in HeLa cervical cancer cell lines
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Neda Eivazi-Arvanagh   , Arezo Chakerzehi , Mahdieh Hemati , Hananeh Zarinnehad , Ali Moradi |
| Shahid Sadoughi University of Medical Sciences , morady2008@gmail.com |
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Abstract: (5126 Views) |
Background: Cell migration plays a principal role in many biological and pathological processes, including embryonic and tumor metastasis. It is well-known that Rac1 from Rho GTPase family is the key regulator of the cell migration. Melittin is a natural peptide in bee venom with apoptotic and anti cancer effects. This study aimed to examine the effect of melittin on Rac1 expression.
Materials and Methods: HeLa cells were cultured in Dulbecco's modified Eagle's medium until they reached 80% confluency, then treated with melittin (0.5 and 1 μg/ml) with the consideration of IC50=1 μg/ml for 6 hours. In the control group the same volume of distilled water, as the melittin solvent, was added. Thereafter, cells were lyzed with buffer and after centrifugation the protein concentration of supernatant was measured using the Bradford method. The amount of Rac1 expression was determined using the Western blotting technique and chemiluminescence. Outcomes were normalized with β-Actin as an internal control and the results were displayed as the percentage against the control.
Results: The Rac1expression compared to the control (100%), in the presence of different concentrations of melittin (0.5 and 1 μg/ml) were 13.88%±1.15% and 1.7%±0.96%, respectively which showed a significant reduction in Rac1 melittin expression (P<0.05).
Conclusion: It can be concluded that melittin can dose-dependently inhibit the Rac1 expression in HeLa cell line. |
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| Keywords: Rac1, Cell migration, Melittin, HeLa cells |
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Full-Text [PDF 272 kb]
(1558 Downloads)
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Type of Study: Research |
Subject:
medicine, paraclinic
Received: 2015/02/26 | Revised: 2015/03/16 | Accepted: 2015/02/26 | Published: 2015/02/26
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