The effect of indomethacin on pentylenetetrazole-induced kindling in male rats
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Faezeh Daghigh , Mahdi Noureddini , Mahdi Takhtefirouzeh , Azhdar Heydari |
Kashan University of Medical Sciences , heydariazh@kaums.ac.ir |
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Abstract: (4101 Views) |
Background: Cyclooxygenase, the rate-limiting enzyme in prostaglandin synthesis, exists as two isoforms, cyclooxygenase-1 and cyclooxygenase-2. Previous studies have shown the protective effect of cyclooxygenase enzyme in the development of convulsion. However, the involvement of Cyclooxygenase-1 in the pathogenesis of epilepsy has not well known. The present study was designed to explore whether indomethacin (a nonspecific COX inhibitor) can affect pentylenetetrazole (PTZ)-induced kindling in rats. Materials and Methods: Kindling, as a chronic model of epilepsy, was inducted by the intraperitoneal injection of PTZ (37.5 mg/kg) every 48 hours (13 times) in all groups. In the pre-treatment groups, intraperitoneal injection of indomethacin (2.5, 5, 10 mg/kg) was done 20 min before each PTZ injection. In sham group, tween (20%) was used as a solvent. Results: Repeated injections of PTZ after 13 sessions significantly induced the kindling in the control group. In pre-treatment groups, indomethacin (2.5, 5, 10 mg/kg) significantly decreased PTZ-induced mean kindling scores. Conclusion: The finding that the indomethacin can partially reverse the kindling mean scores in rat indicates the involvement of cycloogygenase-1 isoform. It seems that cycloogygenase-1 inhibitors could be a useful choice for the treatment of epilepsy. |
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Keywords: Kindling, Cyclooxygenase, Indomethacin, Pentylenetetrazole |
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Full-Text [PDF 274 kb]
(1335 Downloads)
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Type of Study: Research |
Subject:
medicine, paraclinic Received: 2014/08/20 | Revised: 2014/09/13 | Accepted: 2014/08/20 | Published: 2014/08/20
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