:: Volume 18, Issue 2 (Bimonthly 2014) ::
Feyz 2014, 18(2): 159-166 Back to browse issues page
A comparative proteomic study of chondral chondrocytes in osteoarthritis patients with normal chondrocytes
Batol Zamani *, Gholam Reza Khosravi, Mostafa Rezaei-Tavirani, Marzieh Hashemi-Takhonche, Saeed Heidari-Keshel
Kashan University of Medical Sciences , Batol_zamani2007@yahoo.com
Abstract:   (6287 Views)

Background: Osteoarthritis (OA) is a common degenerative disease of joints and the main cause of elder patients' disability. Cartilage damage is a major problem in OA. To investigate the pathogenesis of OA, this study aimed to analyze the differential proteomics of chondrocytes in OA patients compared to normal chondrocytes.

Materials and Methods: Protein extracts of cartilages prepared from 7 patients with end-stage OA, who were candidates for arthroplasty, were compared to 7 samples from normal cartilages. Proteins were analyzed by fluorescent gel electrophoresis (2D-DIGE) method and the differentially expressed proteins were identified by mass spectrometry (MS). Two samples were lost because of tissue insufficiency and the other because of mistransportation.

Results: Among the 25 analyzed proteins, difference was seen for the 8 following proteins: osteonectine, cathepsin, chondroadherin, HSP, clustrine and collagen II, Activin A and carbonic Anhydrase. The levels of osteonectine, cathepsin, clustrine and collagen II were decreased in the OA samples compared to normal chondrocytes. Moreover, the levels of chondroadherin and Activin A were increased in the OA samples compared to normal chondrocytes.

Conclusion: The findings demonstrate the ability of the 2D-DIGE/MS platform to identify proteins with altered expression in chondrocytes of patients with OA. The identification of these proteins may open new lines of research for the pathogenesis of OA.

Keywords: Osteoarthritis, Proteome, Cartilage, Chondrocyte, 2D-DIGE
Full-Text [PDF 599 kb]   (2223 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2014/04/20 | Revised: 2014/04/30 | Accepted: 2014/04/20 | Published: 2014/04/20

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Volume 18, Issue 2 (Bimonthly 2014) Back to browse issues page