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:: Volume 17, Issue 6 (Bimonthly 2014) ::
Feyz 2014, 17(6): 537-544 Back to browse issues page
Comparing the in vitro antifungal activity of various Aloe vera leaf extracts on Aspergillus flavus growth and aflatoxin B1 production
Arash Babaei *, Hadis Tavafi, Milad Manafi, Ameneh Fahimifar
Malayer University , arash_babaei@yahoo.com
Abstract:   (4106 Views)

Background: Due to the wide spread of Aspergillus flavus contamination on the food stuff, its complete growth inhibition is inevitable. Aloe vera has anti-microbial compounds that have significant antifungal and antibacterial activities. The aim of this study was to compare the antifungal activity of acetonic, ethanolic and aqueous extracts of Aloe vera on A. flavus growth and aflatoxin B1 production.

Materials and Methods: In this study, the antifungal activity of acetonic, ethanolic, and aqueous extracts of Aloe vera was evaluated using the agar plate diffusion method. Extracts were tested at different concentrations (0, 102, 103, 104 and 105 microliters /liter). To evaluate the effect of the extract on aflatoxin B1 production, the acetonic extract of Aloe vera was used and the amount of toxin produced was measured using the HPLC method.

Results: The maximum antifungal activity (100%) was observed in 105 microliters /liter of acetonic extract. The inhibition percentages of aflatoxin B1 production at 2,000 and 2 microlitters /liter concentrations in 50 ml of culture medium were 40.94% and 18.14%, respectively.

Conclusion: The acetonic extract of Aloe vera can be more effective than the other solvents to prevent A. flavus growth and it can also reduce the production of aflatoxin B1by A. flavus.

Keywords: Aspergillus flavus, Aflatoxin B1, Antifungal activity, Aloe vera, HPLC
Full-Text [PDF 272 kb]   (1304 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2013/12/1 | Accepted: 2013/12/1 | Published: 2013/12/1
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Babaei A, Tavafi H, Manafi M, Fahimifar A. Comparing the in vitro antifungal activity of various Aloe vera leaf extracts on Aspergillus flavus growth and aflatoxin B1 production. Feyz. 2014; 17 (6) :537-544
URL: http://feyz.kaums.ac.ir/article-1-2104-en.html


Volume 17, Issue 6 (Bimonthly 2014) Back to browse issues page
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