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:: Volume 17, Issue 2 (Bimonthly 2013) ::
Feyz 2013, 17(2): 139-148 Back to browse issues page
Metformin reduces cisplatin-mediated apoptosis in gastric adenocarcinoma cells
Jamileh Salaramoli, Hamidollah Ghaffari, Mansoor Heidari, Vahid Lesan *
University of Tehran , vlesan@ut.ac.ir
Abstract:   (6738 Views)

Background: Metformin, a drug widely used for type 2 diabetes, may also have anti-cancer properties. The purpose of this study was to examine the effect of metformin on cisplatin cytotoxicity in the gastric adenocarcinoma cells line (MKN45).

Materials and Methods: In this study, cells viability and apoptosis were measured using the (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and flow cytometry, respectively. Moreover, the expressions of mammalian target of rapamycin, survivin and AKT genes were evaluated by RT-PCR. All experiments were performed in triplicate.

Results: The results showed that each of metformin and cisplatin separately reduced the viability of cancer cell, but in co-administration, metformin reduced the cytotoxicity of cisplatin. In co-administration, the survivin expression was increased followed by a reduction in cisplatin anti-cancer effect. Therefore, the antagonistic effect of drugs can be associated with survivin expression. The results also revealed that the anti-apoptotic effects of metformin co-administrated with cisplatin are associated with increased AKT expression.

Conclusion: It seems that in gastric cancers, metformin is not an appropriate choice to make cells sensitive to cisplatin and the antagonistic effects of the two drugs should be considered when they prescribed in combination.

Keywords: Metformin, Cisplatin, MKN45 cell line, Survivin, AKT
Full-Text [PDF 679 kb]   (2427 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2013/05/11 | Accepted: 2013/05/15 | Published: 2013/05/15
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Salaramoli J, Ghaffari H, Heidari M, Lesan V. Metformin reduces cisplatin-mediated apoptosis in gastric adenocarcinoma cells. Feyz. 2013; 17 (2) :139-148
URL: http://feyz.kaums.ac.ir/article-1-1913-en.html

Volume 17, Issue 2 (Bimonthly 2013) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
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