Background: An antiseptic property is one of the important properties of silver compounds. For this reason, it is widely produced and used in nano dimensions. The aim of this study was to investigate the antioxidant effects of ethanolic extract of Peganum harmala L. on male mice treated with nanosilver particles.

Materials and Methods: This study was performed on male mice weighing 25-30g. This study included 4 groups: a control group treated with distilled water, a group received 500ppm nanosilver particles and a group received distilled water and fed 20mg/kg/day alcoholic extract of Peganum harmala in 30 days. The last group was fed with an alcoholic extract of Peganum harmala orally and received nanosilver particles with the same dose. interperitoneal injections of nanosilver and distilled water were performed on 3 consecutive days at the beginning of the experiment. After the treatment period, blood sample was collected from the heart. The activity of catalase was determined.

Results: Catalase activity decreased significantly in the nanosilver-treated group (P<0.001), but increased in the group received the alcoholic extract of Peganum harmala in comparison with the nanosilver-treated group (P<0.001).

Conclusion: Antioxidant property of the alcoholic extract of Peganum harmala can be reduced the harmful effects of nanosilver particles.