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:: Volume 16, Issue 6 (Bimonthly 2013) ::
Feyz 2013, 16(6): 522-528 Back to browse issues page
Evaluating the relationship between single-nucleotide polymorphism in the TNF-gene promoter and susceptibility to chronic hepatitis B infection in patients referring to Taleghani hospital in Tehran
Hamed Naghoosi, Sayyed Reza Mohebbi *, Sayyed Mohamad Ebrahim Tahaei, Pedram Azimzadeh, Sara Romani, Shohreh Almasi, Mahsa Khanyaghma, Afsaneh Sharifian, Azar Sanati, Mohammad Reza Zali
Shahid Beheshti University of Medical Sciences , srmohebbi@gmail.com
Abstract:   (4543 Views)

Background: Tumor necrosis factor-alpha (TNF-α), a multifunctional proinflammatory cytokine, plays an essential role in the host immune response to hepatitis B virus (HBV). The aim of this study was to examine the relationship between single-nucleotide polymorphism (SNP) in the TNF- 308 gene locus promoter and the susceptibility to chronic HBV infection.

Materials and Methods: In this case-control study, genomic DNA was extracted from peripheral blood samples of 119 chronically HBV infected patients referred to Tehran Taleghani hospital and 111 healthy controls using the phenol-chloroform method. Afterwards, genotyping was performed by the ARMS-PCR method. Data were analyzed using the Chi-square test.

Results: The frequencies of TNF-α genotypes (GG and GA) 308 on locus were 84.9%, 15.1% and 80.2%, 19.8% in the case and control groups, respectively. No AA genotype was seen in both groups. Moreover, no significant difference was found between the case and control groups.

Conclusion: The genetic capacity for cytokine production in individuals has a major effect on their immune system response. Several SNPs have been identified in the human TNF- α gene promoter the polymorphism at 308 locus, which involves substituting guanine (G) for adenine (A), has been linked to an increased susceptibility to several chronic inflammatory diseases. However, the results of this study indicate that there is no relation between the TNF-α-308 locus SNP and susceptibility to chronic HBV infection in Iranian population.

Keywords: TNF-α, Polymorphism, Chronic hepatitis B
Full-Text [PDF 316 kb]   (1599 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2012/12/19
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Naghoosi H, Mohebbi S R, Tahaei S M E, Azimzadeh P, Romani S, Almasi S, et al . Evaluating the relationship between single-nucleotide polymorphism in the TNF-gene promoter and susceptibility to chronic hepatitis B infection in patients referring to Taleghani hospital in Tehran. Feyz. 2013; 16 (6) :522-528
URL: http://feyz.kaums.ac.ir/article-1-1700-en.html


Volume 16, Issue 6 (Bimonthly 2013) Back to browse issues page
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