:: Volume 16, Issue 4 (Bimonthly 2012) ::
Feyz 2012, 16(4): 311-316 Back to browse issues page
A molecular beacon-based real time PCR assay for quantitative detection of Toxoplasma gondii in rat
Roghayeh Norozi, Abdolhossein Dalimi-Asl *, Mahdi Forozandeh-Moghadam, Fatemeh Ghaffarifar
Tarbiat Modares University , dalimi_a@modares.ac.ir
Abstract:   (5631 Views)

Background: Application of quantitative real time PCR has evolved as a sensitive, specific, and rapid method for the detection of Toxoplasma gondii (T. gondii). The present study aimed to evaluate the efficacy of real time PCR method, using B1 gene, for the diagnosis of toxoplasmosis in the experimentally infected rats.

Materials and Methods: Parasites were cultured in peritoneal cavity of mice and then the DNA was extracted in tachyzoite stage. The B1 gene of T. gondii was amplified by PCR and detected by real time PCR method based on the molecular beacon probe. Finally, real time PCR was evaluated for the quantization of T. gondii in the blood of the experimentally infected rats.

Results: The B1 gene of T. gondii which was successfully amplified by PCR yielded an amplicon with an approximate length of 116 bp. Using this gene was evaluated highly appropriate for the quantization of T. gondii by real time PCR method.

Conclusion: Application of real time PCR method is shown to be highly efficient in terms of sensitivity and rapidity for the detection of B1 gene as well as the quantization of T. gondii in blood of rat.

Keywords: Toxoplasma gondii, Real time PCR, B1 gene, Rat
Full-Text [PDF 341 kb]   (1578 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2012/07/25

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Volume 16, Issue 4 (Bimonthly 2012) Back to browse issues page