Background: Regarding to prevalence of otitis and different report of etiology and resistant drug and inaccessibility of accurate bacterial agents and the rate of antibiotic sensitivity, this study was performed in patients who referred to Matini Hospital of Kashan.

Materials and Methods: A descriptive study was conducted on 50 patients with supperative otitis media that refereed to the Central Laboratory of Kashan University during 2002-2003. All of patients were examined by Otolaryngologist, then refereed to Centeral Laboratory, specimens were collected and cultured on media: eosin Methylen Blue (EMB), blood agar, Thioglycolate, isolated were identified by National Committee for Clinical laboratory Standard (NCCLS), antibiotic sensitivity was determined according to disc diffusion method (Kirby-Bauer). The results were presented by descriptive analysis.

Results: Rate of infection in the 50 cases were determined 56%, the most common age of patients in the both sex were 25-35 year. Pseudomonas Aeroginosa and Staphylococcus Aureus were the most common pathogens (22%), Pseudomonas showed the most rate of antibiotic sensitivity to vancomycin

Conclusion: P. aeroginosa was the major pathogen in patients with suppurative otitis media, gentamycin and ciprofloxacin were determined as effective drugs.