[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 15, Issue 4 (Quarterly 2011) ::
Feyz 2011, 15(4): 322-330 Back to browse issues page
Expression of nestin and nerve growth factors in adipose-derived mesenchymal stem cells
Mohammad Taghi Ghorbanian , Maryam Haji-Ghasem-Kashani , Leili Hossein-Pour , Leila Mirzaiyan
Damghan University , ghorbanian@du.ac.ir
Abstract:   (23133 Views)

Background: Adipose tissue represents an accessible source of mesenchymal stem cells (Adipose tissue derived mesenchymal stem cells-ADSCs). ADSCs could be differentiated into various mesenchymal stem cells (e.g. chondrocytes, adipocytes, osteoblasts, and neuronal lineages). Neurotrophic factors are diffusible polypeptides that have a critical role in survival, proliferation and differentiation of stem cells. This study aimed to evaluate the proliferative capacity and the expression of some neurotrophic factors such as brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), glial cell-derived neurotrophic factor (GDNF), neurotrophin-3 (NT-3), neurotrophin-4 (NT-4), nerve growth factor (NGF) and the expression of nestin in ADSCs.

Materials and Methods: Rat ADSCs were isolated from the subcutaneous adipose tissue using mechanical and enzymatic digestion. These cells were cultured in αMEM supplemented with 10% fetal bovine serum. ADSCs of the third passage were evaluated by immunocytochemistry to detect the cell surface markers of CD90 and nestin. Reverse transcription-polymerase chain reaction (RT-PCR) was used to study the expression of the above-mentioned neurotrophic factors in the ADSCs. Moreover, adipogenic and chondrogenic differentiation of ADSCs were induced in-vitro.

Results: The assessment of ADSCs identity and purity showed that 90% and 80% of the stem cells were positive for CD90 marker and nestin, respectively. According to RT-PCR results, the above-mentioned neurotrophic factors were expressed in these stem cells. Furthermore, a small number of cells were positive for cresyl violet staining.

Conclusion: Adipose tissue contains a stem cell population that seems to be a good multipotential cell candidate for cell replacement therapy.

Keywords: Mesenchymal stem cell, Neurotrophic factors, Nestin
Full-Text [PDF 373 kb]   (3536 Downloads)    
Type of Study: Research | Subject: General
Received: 2011/11/5 | Revised: 2011/11/22 | Published: 2011/12/15
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghorbanian M T, Haji-Ghasem-Kashani M, Hossein-Pour L, Mirzaiyan L. Expression of nestin and nerve growth factors in adipose-derived mesenchymal stem cells . Feyz 2011; 15 (4) :322-330
URL: http://feyz.kaums.ac.ir/article-1-1310-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 15, Issue 4 (Quarterly 2011) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.05 seconds with 46 queries by YEKTAWEB 4645