[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 23, Issue 2 (Bimonthly 2019) ::
Feyz 2019, 23(2): 108-116 Back to browse issues page
Effect of hydroalcoholic extract of Nectaroscordum tripedale on testicular tissue changes in diabetic male rats
Fereshteh Dadfar * , Jamshid Mohammadi , Hosein Roshanfekr
Department of Biology, Tehran, Payame Noor University, I. R. Iran. , fereshtehdadfar2003@yahoo.com
Abstract:   (297 Views)
Background: Medicinal herbs are important in the treatment of diabetes. Studies have shown that diabetes causes reproductive changes in the male population. This study aimed to examine the effect of the hydroalcoholic extract of Nectaroscordum tripedale on testicular tissue changes in diabetic male rat.
Materials and Methods: In this experimental study, 40 male rats were randomly divided into five groups (n=8, each group). The first and third groups (normal control and diabetic control) received distilled water. The second group (sham) received 50 mg/kg hydroalcoholic extract and the fourth and fifth groups (diabetic treatment 1 and 2) received 50 and 100 mg / kg of hydroalcoholic extract of Nectaroscordum tripedale, respectively. Streptozotocin was used to induce diabetes. At the end of the period, after the anesthesia of the rat, the testicular tissue was isolated and tissue sections were prepared for examination of the testicular tissue changes in the experimental groups. Data were analyzed by one-way ANOVA and Tukey's test using the SPSS software.
Results: According to the results of this study, the number of spermatozoa tubes and the number of sperm in the diabetic control group showed a significant decrease compared to normal and control groups (P=0.03). Nectaroscordum tripedale extract increased the number of sperm, and sperm tubes and improved testicular tissues in diabetic rats.
Conclusion: It seems that the extract of Nectaroscordum tripedale has effects on reducing testicular tissue degradation and increasing the amount of sperm in diabetic rats.
Keywords: Diabetes, Nectaroscordum tripedale, Testicular tissue changes, Rat
Full-Text [PDF 607 kb]   (140 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2018/10/6 | Accepted: 2019/03/5 | Published: 2019/06/10
1. Tao Z, Shem A, Zhao J. Epidemiological Perspectives of Diabetes. Cell BiochemBiophys 2015; 73(1): 181-5.
2. Davis SN. Insulin oral hypoglycemic Agent and the Pharmacology of the Endocrine Pancreas. In: Goodman and Gilmans the Pharmacologyical basis of therapeutics. Brunton LL. (Ed). McGRAW-Hill, New York; 2004. p. 1613-445.
3. Vara JJ, Deboer C, Dietz A, Hanka LJ, Sokolski WT. Streptozotocin a new antibacterial antibiotic. Antibiotic Annu 1959-1960; 7: 230-35.
4. Amaral S, Moreno AJ, Santos, MS, Seica R, Ramalho-santos J. Effects of hyperglycemia on sperm and testicular cells of Goto-Kakizaki and streptozotocin-treated rat models for diabetes. Theriogenology 2006; 66(9): 2056-67.
5. Khayatnori MH, Khaki A, Safavi A, Sarafinoori H. Effect of growth hormone on the testis and the index spermiogenesis following administration of methotrexate in rat. J Vet Med (Sanandaj) 2010; 3(9): 77-85.
6. Mosher WD, Pratt WF. Fecundity and infertility in the United States: incidence and trends. Ferti Steril 1991; 56(3): 192-3.
7. Tripathi BK, Srivastava AK. Diabetes complications and therapeutics. Med Sci Monitor 2006; 12(7): 130-47.
8. Bayan L, Koulivand PH, Gorji A. Garlic: a review of potential therapeutic effects. Avicenna J Phytomed 2014; 4: 1–14.
9. Paydar S, Jelodar Gh, Mohammadi J, Mohammadi N.The Effect of Hydroalcoholic Extract of Nectaroscordum Tripedale on Liver and Kidney Functional Parameters in Streptozotocin-induced Diabetic Male Rats. Iran J Endocrinol Metabol 2014, 18: 112-9.
10. Tepe B, Sokmen M, Akpulat HA, Sokmen A. In vitro antioxidant activities of the methanol extracts of five Allium species from Turkey. Food Chem 2005; 92: 89-92.
11. Benkeblia N. Free-radical scavenging capacity and antioxidant properties of some selected onions (Allium cepa L.) extracts. Brazilian Arch Biol Technol 2005; 48: 753-9.
12. Prakash D, Brahma NS, Upadhyay N. Antioxidant and free radical scavenging activities of phenols from onion (Allium cepa). Food Chem 2007; 102: 1389-93.
13. Shon MY, Choi SD, Kahng GG, Nam SH, Sung NJ. Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions. Food Chem Toxicol 2004; 42(4): 659-66.
14. Paydar S, Jelodar GA, Mohammadi J. The Effect of Hydroalcoholic Extract of Nectaroscordum Tripedale on Biochemical Factors in Diabetic Rats. Armaghane-danesh 2015; 20 (5): 404-15. [in Persian]
15. Mirfard M, Johari H, Mokhtari M, Hematkhah V, Jamali H, Allahverdi Gh. The Effect of Hydro-Alcoholic Garlic Extract on Testis Weight and Spermatogenesis in Mature Male Rats under Chemotherapy with Cyclophosphamide. J Fasa Med Univ 2011, 3: 123-29. [in Persian]
16. Kazemian S, Karimi A, Pilevariyan A, Ghandi A. The Effect of Hydro-Alcoholic Shallots Extract on Testis and Spermatogenesis in Balb/C Mice. J Zanjan Med Univ 2017; 25(109): 50-63.
17. Mohammadi J, Naik PR. Antidiabetic effects of Morus alba in experimentally induced diabetes in Wistar rat. Biomedicine 2008; 28: 112-6.
18. Berthold HK, Sudhop T, Vonbergmann K. Effect of garlic oil preparation on serum lipoproteins and cholesterol metabolism: A randomized controlled trial. JAMA 1998; 279: 1900-02.
19. Masjedi F, Gol A, Dabiri SH. Preventive effect of garlic (allium sativum l.) on serum biochemical factors and histopathology of pancreas and liver in streptozotocininduced diabetic rats. Iran J Pharm Res 2013; 12(3): 325–38.
20. Liu CT Wong PL, Lii CK, Hse H, Sheen LY. Antidiabetic effect of garlic oil but not diallyl disulfide in rats with streptozotocin-induced diabetes. Food Chem Toxicol 2006; 44(8): 1377–84.
21. Nishino N, Tamori Y, Kasuga M. Insulin efficiently stores triglycerides in adipocytes by inhibiting lipolysis and repressing PGC-1alpha induction. Kobe J Med Sci 2007; 53(3): 99-106.
22. Jiang GY. Practical diabetes. Beijing: People's Health Publishing House 1996. p. 295.
23. Soudamani S, Yuvaraj S, Rengarajan S, Sivakumar R, Malini T, Balasubramanian K. Effects of streptozotoc in diabetes and insulin replacement on androgen and estrogen receptor concentrations in the epididymis of Wistar rats. JER 2006; 10(1): 59-61.
24. Altay B, Doanavargil B, Hekimgil M, Semerci B. Streptozotocin-induced diabetic effects on spermatogenesis with proliferative cell nuclear antigen immunostaining of adult rat testis. Fertil Steril 2003; 80: 828-31.
25. Balester J, Dominguze J, Carman Munoz M, Meritexell S, Riagau T, Joan J. Insulin depend diabets effects tasticular funtions by FSH and LH-linked mechanism. J Androl 2004; 25(5): 706-19.
26. Abdollah Nejad A, Gol A, Dabiri SH, Javadi A. Effects of garlic juice on diabetes-induced testicular damage in rats. Iran J Endocrinol Metab 2009; 11(4): 443-53. [in Persian]
27. Noori M, Khaki SH, Fathiazar F, Rashidi M. The Protective Effects of Carrot Seed Extract on Spermatogenesis and Cauda Epididymal Sperm Reserves in Gentamicin Treated Rats. Cell J (Yakhteh) 2009; 11(3): 327-33.
28. Agarwal A, Prabakaran SA, Said TM. Prevention of oxidative stress injury to sperm. J Androlol 2005; 26(6): 654-60.
29. Amin A, Hamza AA. Effects of roselle and ginger on cisplatin-induced reproductive toxicity in rast. Asian J Androl 2006; 8(5): 607-12.
30. Popovic M, Kaurinovic B, Trivic S, Mimica-Dukic N, Bursac M. Effect of celery (Apium graveolens) extracts on some biochemical parameters of oxidative stress in mice treated with carbon tetrachloride. Phytotherapy Res 2006; 20: 531-7.
31. Guo-Lian Ding, Ye Liu, Miao-E Liu, Jie-Xue Pan, Meng-Xi Guo, Jian-Zhong Sheng, et al. The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis. Asian J Androl 2015; 17(6): 948–53.
32. Mosher WD, Pratt WF. Fecundity and infertility in the United States incidence and trends. Fertil Steril 1991; 56(2): 192-3.
33. Schoeller EL, Albanna G, Frolova AL, Moley KH. Insulin Rescues Impaired Spermatogenesis via the Hypothalamic-Pituitary-Gonadal Axis in Akita Diabetic Mice and Restores Male Fertility. Diabetes 2012;61:1869-1878.
34. Guyton Arthur C, Hall J. Textbook of medical physiology. 11th ed. Philadelphia Elsevier Saunders; 2006. p. 972-6.
35. Bahrami KH, Mahjor AA, Johary H, Bahrami R, Bahrami A. Comparative Study on Histopatological and Histomorphometric Effect of Raw and Cooked Garlic on Spermatogenesis in Testis and Epidydims of Rats. J Fasa Univ Med Sci 2013; 3: 371-9. [in Persian]
36. Kasuga S, Uda N, Kyo E, Ushijima M, Morihara N, Itakura Y. Pharmacologic activities of aged garlic extract in comparison with other garlic preparations. J Nutr 2001; 131(3): 1080–4.
37. Nikravesh M, Jlali M, Mohammadi SH. Effect of Allium cepa extract on Testis tissue of mal rat. Fertil Steril 2008; 4: 239-44.[in Persian]
38. Nejati V, Khaneshi F. The Effect of Hydroalcoholicextract of Descurainia Sophia Seed on the Changes of Testis Tissue, Sperm Parameters, and Testosterone Level in Rats with Streptozotocin-induced Diabetes. Qom Univ Med Sci J 2014; 8(5): 26-33.
39. Ola-Mudathir KF, Suru SM, Fafunso MA, Obioha UE, Faremi TY. Protective roles of onion and garlic extracts on cadmium-induced changes in sperm characteristics and testicular oxidative damage in rats. Food Chem Toxicol 2008; 46(12): 3604-11.
40. Izawa H, Kohara M, Aizawa K, Suganuma H, Inakuma T, Watanabe G, et al. Alleviative effects of quercetin and onion on male reproductive toxicity induced by diesel exhaust particles. Biosci Biotechnol Biochem 2008; 72(5): 1235-41.
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Dadfar F, Mohammadi J, Roshanfekr H. Effect of hydroalcoholic extract of Nectaroscordum tripedale on testicular tissue changes in diabetic male rats. Feyz. 2019; 23 (2) :108-116
URL: http://feyz.kaums.ac.ir/article-1-3716-en.html

Volume 23, Issue 2 (Bimonthly 2019) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 3937