[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 26, Issue 5 (Bimonthly 2022) ::
Feyz 2022, 26(5): 491-500 Back to browse issues page
The effect of cinnamon (Cinnamomum zeylanicum) extract on modulating the expression of cation-chloride transporters (NKCC1 and KCC2) after stroke in rats receiving high-fat diet
Parvin Hojati, Mehdi Rahnema , Ali Rostami , Hossein Mostafavi *
Department of Physiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, I.R. Iran. , mostafavih@zums.ac.ir
Abstract:   (404 Views)
Background: Cinnamon improves the lipid profile and has antioxidant and protective effects. Therefore, in this study, the effect of cinnamon on the expression of NKCC1 and KCC2 genes in the stroke model of rats receiving a high-fat diet (HFD) was investigated.
Materials and Methods: Thirty-six rats were divided into four groups: control, model (stroke), cinnamon 130 and 260 mg. All groups, except the control group, received HFD for 8 weeks, and then the groups receiving cinnamon were injected with 130 and 260 mg/day of cinnamon for 6 weeks, respectively. The animals were anesthetized and stroke was induced in them with middle cerebral artery occlusion model. After 12 hours, the animals were assessed for body mass index (BMI), stroke volume and expression of NKCC1 and KCC2 genes. One-way ANOVA was used to analyze the data at a significance level of P<0.05.
Results: HFD increased BMI. Cinnamon extract decreased BMI and reduced stroke volume compared to the model group. Decreased expression of NKCC1 and increased expression of KCC2 were significant in stroke hemispheres compared to the model group. The low dose of the extract had a better effect in reducing the expression of genes.
Conclusion: It seems that cinnamon, as a pre-treatment, has valuable and reducing effects on BMI and ischemic strokes caused by obesity, and by modulating the expression of KCC2 and NKCC1 genes, it maintains chlorine ion homeostasis and therefore, it can be considered as a suitable option for the pre-treatment of stroke.
Keywords: Strokes, Cinnamon, Membrane transporters, Ischemia reperfusion injury
Full-Text [PDF 405 kb]   (217 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2022/09/14 | Accepted: 2022/11/5 | Published: 2022/12/4
References
1. Sarikaya H, Ferro J, Arnold M. Stroke prevention--medical and lifestyle measures. Eur Neurol 2015; 73(3-4): 150-7.
2. Lin MP, Liebeskind DS. Imaging of Ischemic Stroke. Continuum (Minneap Minn) 2016; 22(5, Neuroimaging): 1399-423.
3. Martín-Aragón Baudel MA, Poole AV, Darlison MG. Chloride co-transporters as possible therapeutic targets for stroke. J Neurochem 2017; 140(2): 195-209.
4. Mikawa S, Wang C, Shu F, Wang T, Fukuda A, Sato K. Developmental changes in KCC1, KCC2 and NKCC1 mRNAs in the rat cerebellum. Brain Res Dev Brain Res 2002; 136(2): 93-100.
5. Schulte JT, Wierenga CJ, Bruining H. Chloride transporters and GABA polarity in developmental, neurological and psychiatric conditions. Neurosci Biobehav Rev 2018; 90: 260-71.
6. Kipnis PA, Kadam SD. Novel Concepts for the Role of Chloride Cotransporters in Refractory Seizures. Aging Dis 2021; 12(4): 1056-69.
7. Alkahtani R. Molecular mechanisms underlying some major common risk factors of stroke. Heliyon 2022; 8(8): e10218.
8. Wang X, Huang Y, Chen Y, Yang T, Su W, Chen X, et al. The relationship between body mass index and stroke: a systemic review and meta-analysis. J Neurol 2022.
9. Varnaseri M, Rahnema M, Bigdeli MR. The effect of edible oil from Portulaca oleracea seeds on the blood-brain barrier permeability in rat. Feyz 2016; 20(5): 405-12. [in Persian [
10. Rahnema M, Foroozandeh M, E. G. The effect of hydroalcoholic extract of Satureja hortensis on outcomes of stroke in rat. Feyz 2016; 19(6): 511-9. [in Persian [
11. Wang Y, Shi Y, Zou J, Zhang X, Wang M, Guo D, et al. The intranasal administration of Carthamus tinctorius L. extract/phospholipid complex in the treatment of cerebral infarction via the TNF-α/MAPK pathway. Biomed Pharmacother 2020; 130: 110563.
12. Rabiei Z, Bigdeli MR, Rasoulian B, Ghassempour A, Mirzajani F. The neuroprotection effect of pretreatment with olive leaf extract on brain lipidomics in rat stroke model. Phytomedicine 2012; 19(10): 940-6.
13. Silva ML, Bernardo MA, Singh J, de Mesquita MF. Cinnamon as a Complementary Therapeutic Approach for Dysglycemia and Dyslipidemia Control in Type 2 Diabetes Mellitus and Its Molecular Mechanism of Action: A Review. Nutrients 2022; 14(13.(
14. Keramati M, Musazadeh V, Malekahmadi M, Jamilian P, Jamilian P, Ghoreishi Z, et al. Cinnamon, an effective anti-obesity agent: Evidence from an umbrella meta-analysis. J Food Biochem 2022; 46(8): e14166.
15. Angelopoulou E, Paudel YN, Piperi C, Mishra A. Neuroprotective potential of cinnamon and its metabolites in Parkinson's disease: Mechanistic insights, limitations, and novel therapeutic opportunities. J Biochem Mol Toxicol 2021; 35(4): e22720.
16. Zhang L, Li Z, Wu Y, Fan Y, He Z, He P, et al. Cinnamon and Aspirin for Mild Ischemic Stroke or Transient Ischemic Attack: A Pilot Trial. Clin Therapeutics 2022; 44(4): 482-90.
17. Panickar KS, Polansky MM, Anderson RA. Cinnamon polyphenols attenuate cell swelling and mitochondrial dysfunction following oxygen-glucose deprivation in glial cells. Experimental Neurol 2009; 216(2): 420-7.
18. Tuzcu Z, Orhan C, Sahin N, Juturu V, Sahin K. Cinnamon Polyphenol Extract Inhibits Hyperlipidemia and Inflammation by Modulation of Transcription Factors in High-Fat Diet-Fed Rats. Oxidative Med Cellular Longevity 2017; 2017: 1583098.
19. Mostajabi Dastjerdi MR. The effect of Cinnamomum zeylanicum extract pre- nutrition on stroke induced neurological deficits in high fat diet rats [Dissertation] Zanjan. Zanjan university of medical sciences; 2017. [in Persian [
20. Ranasinghe P, Perera S, Gunatilake M, Abeywardene E, Gunapala N, Premakumara S, et al. Effects of Cinnamomum zeylanicum (Ceylon cinnamon) on blood glucose and lipids in a diabetic and healthy rat model. Pharmacognosy Res 2012; 4(2): 73-9.
21. Mostafavi H, Hatami M, Alipour M, Mousavi S.S, H. F. Effect of cinnamon on antioxidant content and ZO-1 gene expression in brain following middle cerebral artery occlusion in rats receiving high-fat diet. Physiol Pharmacol 2022.
22. Briyal S, Sharma U, Jagannathan NR, Gupta YK. Effect of vineatrol in focal cerebral ischemia in rats. Methods Find Exp Clin Pharmacol 2009; 31(8): 505-11.
23. Longa EZ, Weinstein PR, Carlson S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 1989; 20(1): 84-91.
24. Shahjouei S, Cai PY, Ansari S, Sharififar S, Azari H, Ganji S, et al. Middle Cerebral Artery Occlusion Model of Stroke in Rodents: A Step-by-Step Approach. J Vasc Interv Neurol 2016; 8(5): 1-8.
25. Novelli EL, Diniz YS, Galhardi CM, Ebaid GM, Rodrigues HG, Mani F, et al. Anthropometrical parameters and markers of obesity in rats. Laboratory Animals 2007; 41(1): 111-9.
26. Yan Y, Dempsey RJ, Flemmer A, Forbush B, Sun D. Inhibition of Na+–K+–Cl− cotransporter during focal cerebral ischemia decreases edema and neuronal damage. Brain Res 2003; 961(1): 22-31.
27. Mu XP, Wang HB, Cheng X, Yang L, Sun XY, Qu HL, et al. Inhibition of Nkcc1 promotes axonal growth and motor recovery in ischemic rats. Neuroscience 2017; 365: 83-93.
28. Mousavi SM, Rahmani J, Kord-Varkaneh H, Sheikhi A, Larijani B, Esmaillzadeh A. Cinnamon supplementation positively affects obesity: A systematic review and dose-response meta-analysis of randomized controlled trials. Clin Nutr (Edinburgh, Scotland) 2020; 39(1): 123-33.
29. Ogasawara J, Kitadate K, Nishioka H, Fujii H, Sakurai T, Kizaki T, et al. Oligonol, an oligomerized lychee fruit-derived polyphenol, activates the Ras/Raf-1/MEK1/2 cascade independent of the IL-6 signaling pathway in rat primary adipocytes. Biochem Biophys Res Commun 2010; 402(3): 554-9.
30. Van Liefferinge E, Müller M, Van Noten N, Degroote J, Niknafs S, Roura E, et al. Cinnamaldehyde Induces Release of Cholecystokinin and Glucagon-Like Peptide 1 by Interacting with Transient Receptor Potential Ankyrin 1 in a Porcine Ex-Vivo Intestinal Segment Model. Animals (Basel) 2021; 11(8.(
31. Vafa M, Mohammadi F, Shidfar F, Sormaghi MS, Heidari I, Golestan B, et al. Effects of cinnamon consumption on glycemic status, lipid profile and body composition in type 2 diabetic patients. Int J Prev Med 2012; 3(8): 531-6.
32. Sadighi S. The effect of (Cinnamomum zeylanicum L) Extracts pre-nutrition On ischemic tolerance in the rat stroke models [Dissertation]: Islamic Azad University. Zanjan Branch.; 2014. [in Persian.
33. Jurcau A, Simion A. Neuroinflammation in Cerebral Ischemia and Ischemia/Reperfusion Injuries: From Pathophysiology to Therapeutic Strategies. Int J Mol Sci 2021; 23(1).
34. Murcia MA, Egea I, Romojaro F, Parras P, Jiménez AM, Martínez-Tomé M. Antioxidant evaluation in dessert spices compared with common food additives. Influence of irradiation procedure. J Agric Food Chem 2004; 52(7): 1872-81.
35. Dhuley JN. Anti-oxidant effects of cinnamon (Cinnamomum verum) bark and greater cardamom (Amomum subulatum) seeds in rats fed high fat diet. Indian J Exp Biol 1999; 37(3): 238-42.
36. Lalkovičová M, Danielisová V. Neuroprotection and antioxidants. Neural Regen Res 2016; 11(6): 865-74.
37. Xu W, Mu X, Wang H, Song C, Ma W, Jolkkonen J, et al. Chloride Co-transporter NKCC1 Inhibitor Bumetanide Enhances Neurogenesis and Behavioral Recovery in Rats After Experimental Stroke. Mol Neurobiol 2017; 54(4): 2406-14.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hojati P, Rahnema M, Rostami A, Mostafavi H. The effect of cinnamon (Cinnamomum zeylanicum) extract on modulating the expression of cation-chloride transporters (NKCC1 and KCC2) after stroke in rats receiving high-fat diet. Feyz 2022; 26 (5) :491-500
URL: http://feyz.kaums.ac.ir/article-1-4707-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 26, Issue 5 (Bimonthly 2022) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.04 seconds with 29 queries by YEKTAWEB 4549