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:: Volume 21, Issue 4 (Bimonthly 2017) ::
Feyz 2017, 21(4): 352-358 Back to browse issues page
Gene molecular study of biofilm of Staphylococcus aureus isolated from fresh milk using multiplex polymerase chain reaction
Atefeh Mahmoudi-Kojedi , Kumarss Amini
Department of Microbiology, Faculty of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, I. R. Iran. , dr_kumarss_amini@yahoo.com
Abstract:   (1049 Views)
Background: Staphylococcus aureus is one of the main causes of food poisoning in the world. This pathogen has the ability to create biofilms that can lead to food contamination. The presence of biofilm genes in bacteria is very important. The aim of this study was to identify sticky genes (eno, cna, ebp, bbp) that play an important role in virulence and pathogenicity of the bacteria and even prevent the penetration of antibiotics in pathogenicity time.
Materials and Methods: A total of 100 samples of fresh milk were collected from live animals and 60 isolates were selected to identify sticky genes (eno, cna, ebp, bbp) in the production of biofilm of S. aureus using the multiplex polymerase chain reaction method. In addition, the frequency rates of S. aureus strains resistant and susceptible to antibiotics such as methicillin, vancomycin, and clindamycin were determined among the samples.
Results: From a total of 60 isolates of fresh milk, 43.4% of the colonies had laminin-binding protein gene or eno gene. Also, 90% of the isolates were sensitive to vancomycin, 50% sensitive to clindamycin and 43.4% sensitive to methicillin. Distribution rates of other sticky genes including ebp, cna, bbp were 11.6%, 20% and 25%, respectively. Molecular study results showed that the highest and lowest percentages of genes were related to the eno and bbp genes, respectively.
Conclusion: The present study shows that the maximum sensitivity of the samples (90%) was related to vancomycin and the least amount of sensitivity (43.3%) was related to methicillin.
Keywords: Milk, Biofilm, Staphylococcus aureus, Multiplex polymerase chain reaction
Full-Text [PDF 219 kb]   (444 Downloads)    
Type of Study: Research | Subject: General
Received: 2016/12/3 | Accepted: 2017/06/8 | Published: 2017/10/7
1. Fueyo JM, Mendoza MC, Martin MC. Enterotoxins and toxic shock syndrome toxin in Staphylococcus aureus recovered from human nasal carriers and manually handled foods: epidemiological and genetic findings. Microb Infect 2005; 7(2): 187-94.
2. Adwan GM, Abu-Shanab B, Adwan K. Enterotoxigenic Staphylococcus aureus in raw milk in the North of Palestine. Turk J Biol 2006; 29(4): 229-32.
3. Qasemi HR. Iran and Its Policy Against Terrorism. Eradicating Terrorism from the Middle East: Springer; 2016. p. 201-21.
4. Ghasemian A, Najar Peerayeh S, Bakhshi B, Mirzaee M. The microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) genes among clinical isolates of Staphylococcus aureus from hospitalized children. Iran J Pathol 2015; 10(4): 258-64.
5. Arciola CR, Campoccia D, Gamberini S, Baldassarri L, Montanaro L. Prevalence of cna fnbA and fnbB adhesin genes among Staphylococcus aureus isolates from orthopedic infections associated to different types of implant. FEMS Microbiol Letters 2005; 246(1): 81-6.
6. Yarwood JM, Bartels DJ, Volper EM, Greenberg EP. Quorum sensing in Staphylococcus aureus biofilms. J Bacteriol 2004; 186(6): 1838-50.
7. Lina G, Boutite F, Tristan A, Bes M, Etienne J, Vandenesch F. Bacterial competition for human nasal cavity colonization: role of staphylococcal agr alleles. J Appl Environ Microbiol 2003; 69(1): 18-23.
8. Tristan A, Ying L, Bes M, Etienne J, Vandenesch F, Lina G. Use of multiplex PCR to identify Staphylococcus aureus adhesins involved in human hematogenous infections. J Clin Microbiol 2003; 41(9): 4465-7.
9. Maktabi S, Pourmehdi M, Zarei M, Fooladgar AA. Detection of Antibiotic Resistant Listeria spp. in Beef Burgers Distributed in Ahvaz City, Iran. Jundishapur J Health Sci 2016; 8(2(.
10. Bakhshandeh H, Soleimani M, Hosseini SS, Hashemi H, Shabani I, Shafiee A, et al. Poly (epsilon-caprolactone) nanofibrous ring surrounding a polyvinyl alcohol hydrogel for the development of a biocompatible two-part artificial cornea. Int J Nanomedicine 2011; 6: 1509.
11. De S, Brahma B, Polley S, Mukherjee A, Banerjee D, Gohaina M, et al. Simplex and duplex PCR assays for species specific identification of cattle and buffalo milk and cheese. Food Control 2011; 22(5): 690-6.
12. El-Ghodban A, Ghenghesh KS, Marialigeti K, Esahli H, Tawil A. PCR detection of toxic shock syndrome toxin of Staphylococcus aureus from Tripoli, Libya. J Med Microbiol 2006; 55(2): 179-82.
13. Gordon RJ, Lowy FD. Pathogenesis of methicillin-resistant Staphylococcus aureus infection. Clin Infectious Dis 2008; 46(Supplement 5): S350-9.
14. Kuzma K, Malinowski E, Klossowska A, Kaczmarowski M, Smulski S. Detection of adhesin genes by PCR and analysis of their distribution in Staphylococcus aureus strains isolated from bovine mastitis. Bull Vet Inst Pulawy 2006; 50(3): 319.
15. Miller LG, Diep BA. Colonization, fomites, and virulence: rethinking the pathogenesis of community-associated methicillin-resistant Staphylococcus aureus infection. Clin Infect Dis 2008; 46(5): 752-60.
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Mahmoudi-Kojedi A, Amini K. Gene molecular study of biofilm of Staphylococcus aureus isolated from fresh milk using multiplex polymerase chain reaction. Feyz. 2017; 21 (4) :352-358
URL: http://feyz.kaums.ac.ir/article-1-3193-en.html

Volume 21, Issue 4 (Bimonthly 2017) Back to browse issues page
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