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:: Volume 24, Issue 2 (Bimonthly 2020) ::
Feyz Med Sci J 2020, 24(2): 122-132 Back to browse issues page
The effect of combination of Lactobacillus reuteri and Coumarin on the inhibition of gastric cancer cells, AGS cell line
Parisa Fouman-Ajirlou , Changiz Ahmadizadeh * , Mohamad Zaeifizadeh
Department of Microbiology, Ahar Branch, Islamic Azad University, Ahar, I.R. Iran. , dr_ahmadizadeh@yahoo.com
Abstract:   (2623 Views)
Background: Lactobacillus reuteri has broad-spectrum effects on both gram-positive and negative bacteria as well as fungi and protozoa. Also, Coumarins have high ability to control and treat cancer. Apoptosis is a planned cell death that plays an essential role in the development and homostasis of living creatures. Bax and Bcl2 genes are among key target genes of internal pathway; thus the present study aimed to determine the effect of L. reuteri and Coumarin on the expression of these two genes on AGS cell line.
Materials and Methods: After cultivation of gastric cancer cells, Coumarin and L. reuteri were affected in these concentrations at different concentrations and the inhibitory effect on cell proliferation was evaluated using the MTT method. Afterwards, the RNA content of cells was extracted using an RNA extraction kit. Then the cDNA was synthesized for Bax and Bcl2 genes and finally Real time PCR was run.
Results: The results of the MTT assay on gastric cancer cells showed the inhibitory effect of Coumarin and L. reuteri. Also, the results of Real Time PCR showed that the expression of Bax and Bcl2 expression in comparison with the control group was significantly increased.
Conclusion: Considering the effects of Coumarin and L. reuteri on inhibiting proliferation of AGS cells and increasing the Bax and Bcl2 gene, it can be concluded that Coumarin and L. reuteri can induce apoptosis in cancerous cells by affecting the expression of genes involved in apoptosis.
Keywords: Stomach cancer, Lactobacillus reuteri, Coumarin
Full-Text [PDF 445 kb]   (1286 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2019/07/21 | Revised: 2020/07/11 | Accepted: 2020/05/2 | Published: 2020/04/29
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Fouman-Ajirlou P, Ahmadizadeh C, Zaeifizadeh M. The effect of combination of Lactobacillus reuteri and Coumarin on the inhibition of gastric cancer cells, AGS cell line. Feyz Med Sci J 2020; 24 (2) :122-132
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Volume 24, Issue 2 (Bimonthly 2020) Back to browse issues page
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